免疫印迹法的序贯使用以鉴定自身抗体特异性

Sequential use of immunoblots for characterization of autoantibody specificities.

作者信息

Bartsch Holger, Bachmann Michael

机构信息

Institute Immunology, MTZ, Medical Faculty, Carl Gustav Carus University TU Dresden, Fiedlerstrasse 42, D-01307, Dresden, Germany.

出版信息

Methods Mol Biol. 2009;536:293-8. doi: 10.1007/978-1-59745-542-8_31.

DOI:10.1007/978-1-59745-542-8_31

PMID:19378068

Abstract

Sera of patients with systemic autoimmune diseases frequently contain autoantibodies to nuclear autoantigens. Immunoblotting of recombinant and native autoantigens is a commonly used technique for the identification and characterization of autoantibody specificities. Here, we describe an easy procedure that facilitates the comparison of antibody specificities by reusing the same immunoblot at least three times in order to detect an abundantly expressed autoantigen in total cellular extracts.

摘要

患有系统性自身免疫疾病的患者血清中常常含有针对核自身抗原的自身抗体。重组和天然自身抗原的免疫印迹法是用于鉴定和表征自身抗体特异性的常用技术。在此，我们描述了一种简便方法，通过重复使用同一张免疫印迹至少三次来检测总细胞提取物中大量表达的自身抗原，从而便于比较抗体特异性。