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胸膜肺炎放线杆菌9型的溶细胞素

Cytolysins of Actinobacillus pleuropneumoniae serotype 9.

作者信息

Smits M A, Briaire J, Jansen R, Smith H E, Kamp E M, Gielkens A L

机构信息

Department of Molecular Biology, Central Veterinary Institute, Lelystad, The Netherlands.

出版信息

Infect Immun. 1991 Dec;59(12):4497-504. doi: 10.1128/iai.59.12.4497-4504.1991.

DOI:10.1128/iai.59.12.4497-4504.1991
PMID:1937809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC259069/
Abstract

Cytolysin I (ClyI) and cytolysin II (ClyII), which are present in the culture supernatant of Actinobacillus pleuropneumoniae serotype 9, are thought to play an important role in the pathogenesis of pig pleuropneumonia. The purpose of this study was to clone and characterize the genetic determinants of these cytolysins. Cloning was accomplished by the screening of DNA libraries for the presence of cytolytic activity and for the presence of DNA sequences homologous to leukotoxin DNA of Pasteurella haemolytica. Both genetic determinants were found to be members of the RTX cytotoxin family. The ClyII determinant was characterized in more detail. It appeared that ClyII more closely resembled the leukotoxin of P. haemolytica than the alpha-hemolysin of Escherichia coli. The ClyII amino acid sequence was identical to a hemolysin gene sequence of A. pleuropneumoniae serotype 5; this finding indicates that the latter gene also codes for ClyII and not for ClyI, as has previously been suggested. The genetic organization of the ClyII determinant differed from the genetic organization of other RTX determinants. Genes responsible for secretion of ClyII were not contiguous with the toxin gene. Instead, secretion genes were present elsewhere in the genome. These secretion genes, however, belong to the ClyI operon. This indicates that the secretion genes of the ClyI operon are responsible for secretion of ClyI and ClyII.

摘要

细胞溶素I（ClyI）和细胞溶素II（ClyII）存在于胸膜肺炎放线杆菌9型的培养上清液中，被认为在猪胸膜肺炎的发病机制中起重要作用。本研究的目的是克隆并鉴定这些细胞溶素的遗传决定因素。通过筛选DNA文库中是否存在细胞溶解活性以及是否存在与溶血巴斯德菌白细胞毒素DNA同源的DNA序列来完成克隆。发现这两个遗传决定因素均为RTX细胞毒素家族的成员。对ClyII决定因素进行了更详细的鉴定。结果表明，ClyII与溶血巴斯德菌的白细胞毒素比与大肠杆菌的α-溶血素更为相似。ClyII氨基酸序列与胸膜肺炎放线杆菌5型的溶血素基因序列相同；这一发现表明，后一个基因编码的是ClyII而非ClyI，这与之前的推测不同。ClyII决定因素的基因组织与其他RTX决定因素的基因组织不同。负责ClyII分泌的基因与毒素基因不相邻。相反，分泌基因存在于基因组的其他位置。然而，这些分泌基因属于ClyI操纵子。这表明ClyI操纵子的分泌基因负责ClyI和ClyII的分泌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61b9/259069/80d51ddbdbbd/iai00048-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61b9/259069/f0d0331ccd7b/iai00048-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61b9/259069/4e2bcb061632/iai00048-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61b9/259069/18016c466c52/iai00048-0222-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61b9/259069/80d51ddbdbbd/iai00048-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61b9/259069/f0d0331ccd7b/iai00048-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61b9/259069/4e2bcb061632/iai00048-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61b9/259069/18016c466c52/iai00048-0222-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61b9/259069/80d51ddbdbbd/iai00048-0223-a.jpg

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