Heilemann Mike, Hwang Ling Chin, Lymperopoulos Konstantinos, Kapanidis Achillefs N
Department of Physics and IRC in Bionanotechnology, Clarendon Laboratory, University of Oxford, Parks Road, Oxford, OX1 3PU, UK.
Methods Mol Biol. 2009;543:503-21. doi: 10.1007/978-1-60327-015-1_29.
We present a single-molecule method for studying protein-DNA interactions based on fluorescence resonance energy transfer (FRET) and alternating-laser excitation (ALEX) of single diffusing molecules. An application of this method to the study of a bacterial transcription initiation complex is presented.
我们提出了一种基于单扩散分子的荧光共振能量转移（FRET）和交变激光激发（ALEX）来研究蛋白质-DNA相互作用的单分子方法。本文展示了该方法在细菌转录起始复合物研究中的应用。
