Coleman J W, Morris A G
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
Int Arch Allergy Appl Immunol. 1991;94(1-4):179-80. doi: 10.1159/000235356.
We present evidence that a spleen cell-derived factor that inhibits the sensitization of mouse peritoneal mast cells is IFN gamma. Conditioned medium (CM) from Con A-activated mouse spleen cells and recombinant MuIFN gamma both inhibited antigen-induced 5-HT release from peritoneal mast cells when added at the sensitization stage, but were without effect on presensitized cells. Both preparations were active at dilutions corresponding to similar levels of IFN activity (1-10 units/ml). The inhibitory activity of CM was blocked by a rat monoclonal MuIFN gamma-neutralizing antibody, thus confirming that IFN gamma was the active molecule.
我们提供证据表明，一种抑制小鼠腹膜肥大细胞致敏的脾细胞衍生因子是γ干扰素。在致敏阶段添加时，来自刀豆蛋白A激活的小鼠脾细胞的条件培养基（CM）和重组鼠γ干扰素均能抑制抗原诱导的腹膜肥大细胞5-羟色胺释放，但对预先致敏的细胞无作用。两种制剂在对应于相似γ干扰素活性水平（1-10单位/毫升）的稀释度下均具有活性。CM的抑制活性被大鼠单克隆鼠γ干扰素中和抗体阻断，从而证实γ干扰素是活性分子。
