利用微阵列上的夹心酶联免疫吸附测定法对血清抗原进行高通量分析。
High-throughput analysis of serum antigens using sandwich ELISAs on microarrays.
作者信息
Servoss Shannon L, Gonzalez Rachel, Varnum Susan, Zangar Richard C
机构信息
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
出版信息
Methods Mol Biol. 2009;520:143-50. doi: 10.1007/978-1-60327-811-9_10.
Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.
酶联免疫吸附测定（ELISA）微阵列有望成为检测和验证疾病生物标志物的强大工具。ELISA微阵列能够使用少量样本同时检测多种蛋白质。尽管使用ELISA微阵列存在许多潜在的陷阱，但通过精心设计实验可以避免这些问题。在本章中，我们描述了一种用于处理ELISA微阵列的高通量方案，该方案将产生可靠且可重复的数据。
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