一种来自菲律宾的新型乙型肝炎病毒分离株，可能代表一种新的C6亚基因型。

A new isolate of hepatitis B virus from the Philippines possibly representing a new subgenotype C6.

作者信息

Cavinta Lolita, Sun Jianguang, May Anja, Yin Jianhua, von Meltzer Markus, Radtke Monika, Barzaga Nina G, Cao Guangwen, Schaefer Stephan

机构信息

Department of Medical Microbiology, College of Public Health, University of the Philippines Manila, Manila, Philippines.

出版信息

J Med Virol. 2009 Jun;81(6):983-7. doi: 10.1002/jmv.21475.

DOI:10.1002/jmv.21475

PMID:19382274

Abstract

Hepatitis B virus (HBV) genotypes and subgenotypes show distinct geographical prevalence. A genotyping analysis of 28 samples from asymptomatic HBV carriers from the Philippines gave a distribution of HBV genotypes as expected from a previous study: 54% B (15/28), C5 18% (5/28), 14% D (4/28), 7% A1 (2/28). In addition, 7% (2/28) of the samples showed a double infection with genotypes B and D. One of the isolates sequenced completely, ph105, did not group into one of the known subgenotypes after phylogenetic analysis. Ph105 formed a separate clade in genotype C. With a genome length of 3,215 nt. and a serological subtype adr, ph105 exhibited the main features of most genotype C strains. However, ph105 differed by 4.1-7.2% from HBV subgenotypes C1 to C5 when comparing the nucleotide sequence of whole genomes. With only 4.1% difference ph105 was most closely related to subgenotype C2. SimPlot analysis gave no indication for recombination with known HBV genotypes. Ph105 fulfils all criteria for a new subgenotype C6.

摘要

乙型肝炎病毒（HBV）的基因型和亚基因型呈现出不同的地理分布特征。对来自菲律宾无症状HBV携带者的28份样本进行基因分型分析，结果显示HBV基因型的分布与先前研究预期一致：B型占54%（15/28），C5型占18%（5/28），D型占14%（4/28），A1型占7%（2/28）。此外，7%（2/28）的样本显示B型和D型双重感染。经测序的一株分离株ph105，在系统发育分析后未归入已知的亚基因型。Ph105在C基因型中形成一个单独的分支。Ph105基因组长度为3215 nt，血清学亚型为adr，表现出大多数C基因型菌株的主要特征。然而，比较全基因组核苷酸序列时，ph105与HBV亚基因型C1至C5的差异为4.1%-7.2%。Ph105与C2亚基因型的差异仅为4.1%，关系最为密切。SimPlot分析未显示与已知HBV基因型发生重组的迹象。Ph105符合新亚基因型C6的所有标准。