Suzuki Seiji, Sugauchi Fuminaka, Orito Etsuro, Kato Hideaki, Usuda Sadakazu, Siransy Liliane, Arita Isao, Sakamoto Yuko, Yoshihara Namiko, El-Gohary Ahmed, Ueda Ryuzo, Mizokami Masashi
Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Japan.
J Med Virol. 2003 Apr;69(4):459-65. doi: 10.1002/jmv.10331.
The characteristics of hepatitis B virus (HBV) genotype E are not well known because only a few studies have been carried out by complete genome analysis. The aim of this study was to elucidate the distribution of HBV genotypes in Cote d'Ivoire, and to clarify the genotype-related characteristics of genotype E. The distribution of HBV genotypes among 48 HBV carriers in Cote d'Ivoire was determined using serological and genetic methods. The characteristics of genotype E were evaluated by complete genome sequences, and further investigations of small S gene, basic core promoter (BCP) mutation, and precore mutation were undertaken. HBV genotype distribution among the 48 carriers was 6.3% for genotype A, 6.3% for genotype D, and 87.4% for genotype E. Complete genomes of two genotype E strains were sequenced, and found to have 98.2% to 99.2% homology at the nucleotide level when compared with genotype E strains reported previously. In 24 genotype E carriers, the precore mutation was detected in 75% of the patients without HBeAg, in contrast to only 25% of the patients with HBeAg (P < 0.05). All 24 strains have T at nucleotide 1858 in the precore region. In contrast, BCP double mutation was detected in 17% of the patients with HBeAg, and 33% of the patients without HBeAg. These results indicated as the following: (1) genotypes A, D, and E of HBV exist in Cote d'Ivoire and genotype E is the most prevalent; (2) genotype E spread with low genetic diversity over the complete genome in West Africa; (3) HBV precore and/or BCP double variants were common among the patients with genotype E infections.
由于仅有少数研究通过完整基因组分析开展，乙肝病毒（HBV）E基因型的特征尚不为人熟知。本研究的目的是阐明HBV基因型在科特迪瓦的分布情况，并明确E基因型与基因型相关的特征。采用血清学和遗传学方法确定了科特迪瓦48名HBV携带者中HBV基因型的分布。通过完整基因组序列评估E基因型的特征，并对小S基因、基本核心启动子（BCP）突变和前核心突变进行了进一步研究。48名携带者中HBV基因型分布为：A基因型占6.3%，D基因型占6.3%，E基因型占87.4%。对两株E基因型毒株的完整基因组进行了测序，与先前报道的E基因型毒株相比，在核苷酸水平上发现它们具有98.2%至99.2%的同源性。在24名E基因型携带者中，75%的HBeAg阴性患者检测到前核心突变，而HBeAg阳性患者中只有25%检测到（P<0.05）。所有24株毒株在前核心区核苷酸1858处均为T。相比之下，17%的HBeAg阳性患者和33%的HBeAg阴性患者检测到BCP双突变。这些结果表明：（1）HBV的A、D和E基因型存在于科特迪瓦，且E基因型最为普遍；（2）E基因型在西非完整基因组上以低遗传多样性传播；（3）HBV前核心和/或BCP双变异在E基因型感染患者中很常见。
