Buchgraber Manuela, Wasik Andrzej
European Commission, Directorate-General Joint Research Centre, Institute for Reference Materials and Measurements, Food Safety and Quality Unit, Retieseweg 111, B-2440 Geel, Belgium.
J AOAC Int. 2009 Jan-Feb;92(1):208-22.
An interlaboratory trial was conducted to validate an analytical method based on high-performance liquid chromatographic analysis with evaporative light-scattering detection for the simultaneous determination of 9 intense sweeteners, i.e., acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin, and sucralose in carbonated and noncarbonated soft drinks and canned or bottled fruits. Seven laboratories participated in the validation study. The majority of the samples fortified with levels close to the limit of quantification had relative standard deviation for reproducibility (RSDR) values <15%. In most cases, the recovery rates ranged between 90 and 105%, demonstrating satisfactory performance of the method. For samples fortified at levels comparable to the prescribed legal limits stipulated in the current European Union legislation, the method produces acceptably accurate, repeatable, and reproducible results. Trueness, expressed in terms of recovery rates, was demonstrated in most cases by values ranging from 90 to 108%. Comparability of results obtained by individual testing laboratories was good (RSDR values <10%) for the majority of results. Moreover, HorRat values of <1.1 suggested good performance of the method for all sweeteners and matrixes tested.
开展了一项实验室间试验，以验证一种基于高效液相色谱分析和蒸发光散射检测的分析方法，用于同时测定碳酸饮料和非碳酸软饮料以及罐装或瓶装水果中的9种高强度甜味剂，即乙酰磺胺酸钾、阿力甜、阿斯巴甜、环己基氨基磺酸、甘素、纽甜、新橙皮苷二氢查耳酮、糖精和三氯蔗糖。七个实验室参与了验证研究。大多数添加水平接近定量限的样品，其重复性相对标准偏差（RSDR）值<15%。在大多数情况下，回收率在90%至105%之间，表明该方法性能良好。对于添加水平与欧盟现行法规规定的法定限量相当的样品，该方法产生的结果具有可接受的准确性、可重复性和再现性。以回收率表示的准确性，在大多数情况下，其值范围为90%至108%。对于大多数结果，各个检测实验室获得的结果可比性良好（RSDR值<10%）。此外，<1.1的HorRat值表明该方法对所有测试的甜味剂和基质均具有良好性能。
