Bayani Jane, Paliouras Miltiadis, Planque Chris, Shan Shannon J C, Graham Cassandra, Squire Jeremy A, Diamandis Eleftherios P
Department of Applied Molecular Oncology, Ontario Cancer Institute, Princess Margaret Hospital, University Health Network, Toronto, Ontario, M5G 2M9, Canada.
Mol Oncol. 2008 Oct;2(3):250-60. doi: 10.1016/j.molonc.2008.07.001. Epub 2008 Jul 22.
The tissue kallikrein (KLK) genes are a new source for biomarkers in ovarian cancer. However, there has been no systematic analysis of copy number and structural rearrangements related to their protein expression. Chromosomal rearrangements and copy number changes of the KLK region were studied by FISH with protein levels measured by ELISA. Ovarian cancer and cell lines revealed the KLK region was subject to copy number imbalances or involved in unbalanced translocations and were associated with increased protein expression of KLKs 5, 6, 7, 8, 9, 10 and 11. In this initial study, we introduce the potential for long-range chromosomal effects and copy number as a mechanism for the previously reported aberrant expression of many KLK genes in ovarian cancers.
组织激肽释放酶(KLK)基因是卵巢癌生物标志物的新来源。然而,尚未对与其蛋白质表达相关的拷贝数和结构重排进行系统分析。通过荧光原位杂交(FISH)研究KLK区域的染色体重排和拷贝数变化,并用酶联免疫吸附测定(ELISA)测量蛋白质水平。卵巢癌组织和细胞系显示,KLK区域存在拷贝数失衡或参与不平衡易位,并且与KLK 5、6、7、8、9、10和11的蛋白质表达增加有关。在这项初步研究中,我们介绍了长距离染色体效应和拷贝数作为卵巢癌中许多KLK基因先前报道的异常表达机制的可能性。