激肽释放酶基因 10 的过表达是预测胃癌不良预后的生物标志物。
Overexpression of kallikrein gene 10 is a biomarker for predicting poor prognosis in gastric cancer.
机构信息
Xin Jiao, Hai-Ning Zhi, Xiao-Man Liu, Chen-Hao Liu, Department of General Surgery, the First Central Clinical School, Tianjin Medical University, Tianjin 300192, China.
出版信息
World J Gastroenterol. 2013 Dec 28;19(48):9425-31. doi: 10.3748/wjg.v19.i48.9425.
AIM
To analyze the expression of kallikrein gene 10 (KLK10) in gastric cancer and to determine whether KLK10 has independent prognostic value in gastric cancer.
METHODS
We studied KLK10 expression in 80 histologically confirmed gastric cancer samples using real-time quantitative reverse transcription-PCR and hK10 expression using immunohistochemistry. Correlations with clinicopathological variables (lymph node metastasis, depth of invasion and histology) and with outcomes (disease-free survival and overall survival) during a median follow-up period of 31 mo were assessed. Gastric cancer tissues were then classified as KLK10 positive or negative.
RESULTS
KLK10 was found to be highly expressed in 57/80 (70%) of gastric cancer samples, while its expression was very low in normal gastric tissues. Positive relationships between KLK10 expression and lymph node metastasis (P = 0.048), depth of invasion (P = 0.034) and histology (P = 0.015) were observed. Univariate survival analysis revealed that gastric cancer patients with positive KLK10 expression had an increased risk for relapse/metastasis and death (P = 0.005 and 0.002, respectively). Cox multivariate analysis indicated that KLK10 was an independent prognostic indicator of disease-free survival and overall survival in patients with gastric cancer.
CONCLUSION
KLK10 expression is an independent biomarker of unfavorable prognosis in patients with gastric cancer.
目的
分析激肽释放酶基因 10（KLK10）在胃癌中的表达情况，并确定 KLK10 在胃癌中的表达是否具有独立的预后价值。
方法
我们使用实时定量逆转录聚合酶链反应（qRT-PCR）检测 80 例组织学确诊的胃癌样本中 KLK10 的表达情况，并使用免疫组织化学方法检测 hK10 的表达情况。评估 KLK10 表达与临床病理变量（淋巴结转移、浸润深度和组织学）以及中位随访 31 个月期间的无病生存和总生存的相关性。然后将胃癌组织分为 KLK10 阳性或阴性。
结果
我们发现 80 例胃癌样本中，有 57/80（70%）的样本中 KLK10 表达较高，而正常胃组织中其表达非常低。KLK10 表达与淋巴结转移（P=0.048）、浸润深度（P=0.034）和组织学（P=0.015）呈正相关。单因素生存分析显示，KLK10 阳性的胃癌患者复发/转移和死亡的风险增加（P=0.005 和 0.002）。Cox 多因素分析表明，KLK10 是胃癌患者无病生存和总生存的独立预后指标。
结论
KLK10 表达是胃癌患者不良预后的独立生物标志物。
Zotero 插件