Xie Weihua, Sun Chao, Liu Shumin
College of Animal Sciences and Technology, Northwest A&F University, YangLing, ShanXi 712100, China.
Zhongguo Zhong Yao Za Zhi. 2009 Jan;34(2):224-9.
In order to investigate the possible mechanism of its function to degrade lipid, we detect the effects of hawthorn flavanone to the influence on blood-fat levels and adipogenesis genes transcription expression in fat and muscle tissue of hyperlipoidemia mouse.
In this experiment, a total of 48 mouse were randomised to four groups and irrigated with two different concentrations (1.5 g kg(-1) body weight and 3.0 g kg(-1) body weight) of hawthorn flavanone, and killed in 0 h, 1 h, 2 h and 4 h. To estimate the content of TC, TG and HCL-C in blood: Total RNA was isolated from adipose and muscle, Real-time RT-PCR was used to analyze expression changes of adipogenesis genes (SREBP-1c, FAS, HSL and TGH) with time series; to analyze the correlation between TG in blood and some kinds of adipogenesis genes and the ratio of FAS/HARMEAN (HSL, TGH) mRNA in adipose.
Hawthorn flavanone was able to cut down the level ofTC, TG and HDL significantly in blood and achieved the lowest level at 1 h. In adipose tissue, hawthorn flavanone up-regulated FAS, HSL and TGH, and achieved the level of significance (P<0.05), the expression level of FAS and TGH was ascend after 1 h, but HSL descend. The expression level of SREBP-1c was descend rapidly and achieved the level of significance after treating with hawthorn flavanone at 1 h (P<0.05), after that it rise again to even higher than the level of before treatment. After treating with hawthorn flavanone, the ratio of FAS/HARMEAN (HSL, TGH) in adipose was significantly descend and achieved the lowest level at 1 h (P<0.01), but it was descendsubsequently. In muscle tissue, hawthorn flavanone was able to significantly up-regulated the expression of FAS and HSL and lower dose group showed greater increasing, the change of SREBP-1c was similar in adipose tissue except the more heavily upgrade.
Hawthorn flavanone had the function of depressing the concentration of blood-fat, it co-adjusted lipid metabolism of animal by regulating the transcription expression of FAS, HSL, TGH and SREBP-1c especially HSL and SREBP-1c transcription level.
为探讨山楂黄酮降血脂作用的可能机制，检测山楂黄酮对高脂血症小鼠血脂水平及脂肪和肌肉组织中脂肪生成相关基因转录表达的影响。
本实验将48只小鼠随机分为4组，分别灌胃两种不同浓度（1.5 g·kg⁻¹体重和3.0 g·kg⁻¹体重）的山楂黄酮，并在0 h、1 h、2 h和4 h处死。检测血液中总胆固醇（TC）、甘油三酯（TG）和高密度脂蛋白胆固醇（HDL-C）含量：从脂肪和肌肉中提取总RNA，采用实时荧光定量逆转录聚合酶链反应（Real-time RT-PCR）分析脂肪生成相关基因（固醇调节元件结合蛋白-1c，SREBP-1c；脂肪酸合成酶,FAS；激素敏感脂肪酶，HSL；甘油三酯脂肪酶，TGH）随时间的表达变化；分析血液中TG与脂肪生成相关基因及脂肪组织中FAS/HARMEAN（HSL、TGH）mRNA比值的相关性。
山楂黄酮能显著降低血液中TC、TG和HDL水平，在1 h时达到最低水平。在脂肪组织中，山楂黄酮上调FAS、HSL和TGH表达，并达到显著水平（P<0.05），FAS和TGH表达水平在1 h后升高，但HSL下降。SREBP-1c表达水平迅速下降，在1 h用山楂黄酮处理后达到显著水平（P<0.05），之后又上升至高于处理前水平。用山楂黄酮处理后，脂肪组织中FAS/HARMEAN（HSL、TGH）比值显著下降，在1 h时达到最低水平（P<0.01），但随后又下降；在肌肉组织中，山楂黄酮能显著上调FAS和HSL表达，低剂量组升高幅度更大；SREBP-1c变化与脂肪组织相似，但上调更明显。
山楂黄酮具有降低血脂浓度的作用，通过调节FAS、HSL、TGH和SREBP-1c特别是HSL和SREBP-1c转录水平共同调节动物脂质代谢。
