Jiang D F, Zhang X G, Yang H L, Sun C
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Genet Mol Res. 2013 May 13;12(2):1501-10. doi: 10.4238/2013.January.4.13.
Evodiamine is the main active alkali of Wu Zhuyu, a widely distributed Chinese herb. It plays an important role in the regulation of body fat deposition. In this study, we aimed to investigate the effect of evodiamine administration on the expression of genes involved in lipid metabolism in the liver and adipose tissue. Fasted mice were subcutaneously injected with evodiamine (37 °C, 20 mg/kg), and the core body temperature change and expression levels of lipid metabolism-related genes were evaluated at baseline, 0.5, 1, and 2 h. We detected the mRNA expression of fatty acid synthesis enzyme (FAS), peroxisome proliferator-activated receptor gamma (PPAR-γ), sterol regulatory element binding protein 1c (SREBP-1c), triglyceride hydrolase (TGH), and hormone-sensitive lipase (HSL) by real-time PCR and analyzed their correlation with core body temperature. Our results showed that the core body temperature was reduced greater than 1 °C with evodiamine treatment at 1 and 2 h (P < 0.01). In mouse livers, SREBP-1c, HSL, and TGH mRNA expression was significantly increased, and they reached the highest levels 1 h after injection (P < 0.01). However, PPAR-γ mRNA expression was decreased and reached a significant level at 0.5 h (P < 0.01) and FAS mRNA expression was not significantly different; FAS and SREBP-1c mRNA expression were reduced and reached significant levels at 1 h (P < 0.01). Of note, other genes demonstrated opposite changes in adipose tissue, and HSL mRNA expression was significantly reduced at 0.5 h (P < 0.01). The decreasing core temperature had a significant negative correlation with the expression of TGH, HSL, FAS, and SREBP- 1c mRNA in the liver (P < 0.01), but had significant positive correlation with levels of FAS and SREBP-1c mRNA in adipose tissue (P < 0.01). In light of these results, the main mechanism of the regulation of body fat deposition by evodiamine is raising energy consumption through reducing body temperature and promoting fat decomposition.
吴茱萸碱是广泛分布的中草药吴茱萸的主要活性生物碱。它在调节机体脂肪沉积中发挥重要作用。在本研究中，我们旨在探究给予吴茱萸碱对肝脏和脂肪组织中参与脂质代谢的基因表达的影响。对禁食小鼠皮下注射吴茱萸碱（37℃，20mg/kg），并在基线、0.5、1和2小时评估核心体温变化以及脂质代谢相关基因的表达水平。我们通过实时PCR检测脂肪酸合成酶（FAS）、过氧化物酶体增殖物激活受体γ（PPAR-γ）、固醇调节元件结合蛋白1c（SREBP-1c）、甘油三酯水解酶（TGH）和激素敏感性脂肪酶（HSL）的mRNA表达，并分析它们与核心体温的相关性。我们的结果表明，在1小时和2小时给予吴茱萸碱处理后，核心体温降低超过1℃（P<0.01）。在小鼠肝脏中，SREBP-1c、HSL和TGH的mRNA表达显著增加，且在注射后1小时达到最高水平（P<0.01）。然而，PPAR-γ的mRNA表达降低，并在0.5小时达到显著水平（P<0.01），而FAS的mRNA表达无显著差异；FAS和SREBP-1c的mRNA表达在1小时降低并达到显著水平（P<0.01）。值得注意的是，其他基因在脂肪组织中表现出相反的变化，HSL的mRNA表达在0.5小时显著降低（P<0.01）。核心体温降低与肝脏中TGH、HSL、FAS和SREBP-1c的mRNA表达呈显著负相关（P<0.01），但与脂肪组织中FAS和SREBP-1c的mRNA水平呈显著正相关（P<0.01）。鉴于这些结果，吴茱萸碱调节机体脂肪沉积的主要机制是通过降低体温和促进脂肪分解来提高能量消耗。
