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[肉苁蓉种子成分及活力分析的红外光谱研究]

[Infrared spectroscopic study on the component and vigor analysis of Cistanche deserticola seeds].

作者信息

Xu Rong, Sun Su-Qin, Chen Jun, Chen Shi-Lin, Zhou Feng

机构信息

The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Jan;29(1):97-101.

Abstract

Comparative study of the different parts of cistanche deserticola seeds and their changes after different processing were examined by Fourier transform infrared spectroscopy spectra (FTIR). The results of the analysis showed that components in the cistanche deserticola seeds were abundant, which contained characteristic absorption peaks of protein, fat and carbohydrate. As well, pectin and aromatic compound can be also found in the seeds. However, the components were different in different parts of cistanche deserticola seeds. The characteristic absorption peak intensities of fat at 2,926, 1,746, 1,161 and 721 cm(-1) were the strongest in the seed kernels. However, the seed coats mainly consisted of carbohydrate and pectin, which were showed at 1,054 cm(-1). The contents of protein and carbohydrate were decreased distinctly in the moldy and dead seeds after processing. The characteristic absorption peak intensity ratio of protein to fat (I1,630/I1,745 ) was all higher than 1.05 in the live seeds. The characteristic absorption peak intensity ratio of amido link I of protein to fat (11,653/I1,745) in the dead seed kernels of the cistanche deserticola was decreased from 0.31 to 0. 23, which was 25.8% less than that in vital seed kernels. The results suggest that FTIR not only can be used in fast comprehensive analysis of seed components, but also can be used in the seed vigor analysis, seed longevity determination and seed quality evaluation.

摘要

采用傅里叶变换红外光谱(FTIR)对肉苁蓉种子不同部位及其不同加工处理后的变化进行了比较研究。分析结果表明,肉苁蓉种子中的成分丰富,含有蛋白质、脂肪和碳水化合物的特征吸收峰。此外,种子中还可发现果胶和芳香化合物。然而,肉苁蓉种子不同部位的成分有所不同。在种仁中,2926、1746、1161和721cm-1处脂肪的特征吸收峰强度最强。然而,种皮主要由碳水化合物和果胶组成,在1054cm-1处有显示。加工后的霉变种子和死种子中蛋白质和碳水化合物的含量明显降低。活种子中蛋白质与脂肪的特征吸收峰强度比(I1630/I1745)均高于1.05。肉苁蓉死种仁中蛋白质酰胺键I与脂肪的特征吸收峰强度比(11653/I1745)从0.31降至0.23,比活种仁低25.8%。结果表明,FTIR不仅可用于种子成分的快速综合分析,还可用于种子活力分析、种子寿命测定和种子质量评价。

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