Pieters R, Huismans D R, Loonen A H, Hählen K, Veerman A J
Free University Hospital, Department of Pediatrics, Amsterdam, The Netherlands.
Jpn J Cancer Res. 1991 Sep;82(9):1051-5. doi: 10.1111/j.1349-7006.1991.tb01942.x.
We studied whether isotretinoin potentiated the effects of vincristine (VCR), daunorubicin (DNR), and 6-thioguanine (6-TG) against cells obtained from 24 patients with acute lymphoblastic leukemia (ALL). Treatment with 5 micrograms/ml isotretinoin alone resulted in a leukemic cell survival of 82% +/- 28.1%. So isotretinoin is toxic to ALL cells. Dose-response curves were obtained for VCR, DNR and 6-TG in the presence and absence of isotretinoin Isotretinoin showed additive leukemic cell kills in combination with VCR and DNR. When corrected for cell kill by isotretinoin alone, it appeared that isotretinoin did not significantly enhance leukemic cell kills by VCR, DNR and 6-TG. No differences were found between samples from patients at initial diagnosis and at relapse with respect to cell kill by isotretinoin alone and with respect to a possible synergistic effect of isotretinoin and the cytostatic drugs. It is concluded that isotretinoin has additive antileukemic effects in combination with VCR or DNR. However, isotretinoin does not potentiate the antileukemic effects of VCR, DNR and 6-TG against leukemic cells obtained from patients with ALL.
我们研究了异维A酸是否能增强长春新碱（VCR）、柔红霉素（DNR）和6-硫鸟嘌呤（6-TG）对24例急性淋巴细胞白血病（ALL）患者细胞的作用。单独使用5微克/毫升异维A酸治疗导致白血病细胞存活率为82%±28.1%。因此，异维A酸对ALL细胞有毒性。在有和没有异维A酸的情况下，获得了VCR、DNR和6-TG的剂量反应曲线。异维A酸与VCR和DNR联合使用时显示出相加的白血病细胞杀伤作用。当校正单独使用异维A酸的细胞杀伤作用时，似乎异维A酸并没有显著增强VCR、DNR和6-TG对白血病细胞的杀伤作用。在初始诊断和复发时患者的样本中，关于单独使用异维A酸的细胞杀伤作用以及异维A酸与细胞抑制药物可能的协同作用，未发现差异。得出的结论是，异维A酸与VCR或DNR联合使用具有相加的抗白血病作用。然而，异维A酸并不能增强VCR、DNR和6-TG对ALL患者白血病细胞的抗白血病作用。
