Wang Y-Q, Puntenney S B, Burton J L, Forsberg N E
Department of Animal Sciences, Oregon State University, Corvallis, OR 97331, USA.
J Anim Physiol Anim Nutr (Berl). 2009 Feb;93(1):66-75. doi: 10.1111/j.1439-0396.2007.00780.x.
Objectives were to investigate mechanisms by which a nutritional supplement alters immunity in dairy cattle. Our hypothesis was that feeding this product to dairy cattle altered neutrophil gene expression. Eight periparturient Jersey cattle were randomly assigned to one of two treatments: control and treated. Control animals were fed a dry cow ration for 1 month prior to calving. The treated cows were fed the same ration supplemented with OmniGen-AF. Following calving, blood samples were taken and neutrophils were prepared after which RNA was extracted. Gene expression in neutrophils of treated versus control-fed animals was then assessed using bovine-total leukocyte (BOTL-5) arrays. Eighteen genes were differentially regulated in the experimental group and of these, twice as many were up-regulated as down-regulated. Patterns of changes indicated that the additive might alter neutrophil apoptosis, signaling and sensitivity. Two of the regulated genes [interleukin-1beta converting enzyme (ICE) and interleukin-4 receptor (IL-4R)] were investigated in more detail using quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR). Each was found to be elevated by the feeding of experimental product. Increased expression of ICE indicates potential for enhanced neutrophil expression of interleukin-1beta (IL-1beta), a cytokine which plays roles in the inflammatory response and which stimulates adaptive immunity following innate immune activation. Altered expression of IL-4R indicates potential for changes in neutrophil apoptosis. The experiment identified mechanisms by which the additive altered neutrophil gene expression. While many nutrients support the immune system, we have shown that a non-traditional nutritional approach may also have utility in modulating immune function.
目的是研究一种营养补充剂改变奶牛免疫力的机制。我们的假设是，给奶牛喂食这种产品会改变中性粒细胞的基因表达。八头围产期泽西牛被随机分配到两种处理方式之一：对照组和处理组。对照组动物在产犊前1个月喂食干奶牛日粮。处理组奶牛喂食添加了OmniGen - AF的相同日粮。产犊后采集血样并制备中性粒细胞，之后提取RNA。然后使用牛全白细胞（BOTL - 5）阵列评估处理组与对照组喂养动物中性粒细胞中的基因表达。实验组中有18个基因受到差异调节，其中上调的基因数量是下调基因数量的两倍。变化模式表明该添加剂可能会改变中性粒细胞的凋亡、信号传导和敏感性。使用定量逆转录 - 聚合酶链反应（QRT - PCR）对其中两个受调节的基因[白细胞介素 - 1β转换酶（ICE）和白细胞介素 - 4受体（IL - 4R）]进行了更详细的研究。发现每种基因在喂食实验产品后均升高。ICE表达的增加表明中性粒细胞表达白细胞介素 - 1β（IL - 1β）的潜力增强，白细胞介素 - 1β是一种在炎症反应中起作用且在先天免疫激活后刺激适应性免疫的细胞因子。IL - 4R表达的改变表明中性粒细胞凋亡可能发生变化。该实验确定了添加剂改变中性粒细胞基因表达的机制。虽然许多营养素支持免疫系统，但我们已经表明，一种非传统的营养方法在调节免疫功能方面也可能具有效用。
