绝经后女性血清核因子-κB 受体活化因子配体、骨保护素、高敏 C 反应蛋白与骨密度之间的关系：骨免疫与骨炎症

Relationships among serum receptor of nuclear factor-kappaB ligand, osteoprotegerin, high-sensitivity C-reactive protein, and bone mineral density in postmenopausal women: osteoimmunity versus osteoinflammatory.

作者信息

Nabipour Iraj, Larijani Bagher, Vahdat Katayoun, Assadi Majid, Jafari Seyed Mojtaba, Ahmadi Esmaeil, Movahed Ali, Moradhaseli Farzad, Sanjdideh Zahra, Obeidi Narges, Amiri Zahra

机构信息

Department of Endocrine and Metabolic Diseases, The Persian Gulf Tropical Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, Islamic Republic of Iran.

出版信息

Menopause. 2009 Sep-Oct;16(5):950-5. doi: 10.1097/gme.0b013e3181a181b8.

DOI:10.1097/gme.0b013e3181a181b8

PMID:19387415

Abstract

OBJECTIVE

The aim of this study was to investigate the correlations among circulating osteoprotegerin (OPG), the receptor activator of nuclear factor-kappaB ligand (RANKL), high-sensitivity C-reactive protein (hsCRP), and bone mineral density (BMD) in healthy postmenopausal women.

METHODS

In a population-based study, highly specific enzyme-linked immunosorbent assay methods were used to evaluate the sera of 382 healthy Iranian postmenopausal women (mean age +/- SD, 58.7 +/- 7.5 y) for RANKL, OPG, hsCRP, degradation products of C-terminal telopeptides of type I collagen, and osteocalcin. BMD was determined for the lumbar spine (L2-L4) and the proximal femur using dual-energy x-ray absorptiometry.

RESULTS

Circulating levels of OPG (r = 0.30, P < 0.001) and the RANKL/OPG ratio (r = -0.17, P < 0.001) were significantly associated with age. The geometric mean of hsCRP was 1.89 mg/L (SE, 1.05) in the population studied. There was a significant correlation between log(hsCRP) levels and body mass index (BMI; r = 0.36, P < 0.001). Multivariate linear analyses revealed that age (beta = -0.295, P < 0.001), BMI (beta = 0.464, P < 0.001), RANKL (beta = -0.105, P = 0.014), and OPG (beta = 0.098, P = 0.029) were the independent determinants for lumbar BMD (R(2) = 0.35). Age (beta = -0.250, P < 0.001), BMI (beta = 0.486, P < 0.001), and RANKL (beta = -0.110, P = 0.009) were independently correlated with femoral neck BMD (R(2) = 0.36). Age- and BMI-adjusted analysis by quartiles of log-transformed hsCRP did not reveal an association with BMD, serum levels of biochemical markers of bone turnover, RANKL, or OPG.

CONCLUSIONS

The circulating levels of the RANKL/OPG osteoimmunity system have an association with BMD, but subclinical systemic inflammation may not be involved in bone mass in healthy postmenopausal women.

摘要

目的

本研究旨在调查健康绝经后女性循环骨保护素（OPG）、核因子κB受体活化因子配体（RANKL）、高敏C反应蛋白（hsCRP）和骨密度（BMD）之间的相关性。

方法

在一项基于人群的研究中，采用高特异性酶联免疫吸附测定方法，对382名健康的伊朗绝经后女性（平均年龄±标准差，58.7±7.5岁）的血清进行RANKL、OPG、hsCRP、I型胶原C端肽降解产物和骨钙素的评估。使用双能X线吸收法测定腰椎（L2-L4）和股骨近端的骨密度。

结果

OPG的循环水平（r = 0.30，P < 0.001）和RANKL/OPG比值（r = -0.17，P < 0.001）与年龄显著相关。在所研究人群中，hsCRP的几何平均值为1.89 mg/L（标准误，1.05）。log(hsCRP)水平与体重指数（BMI；r = 0.36，P < 0.001）之间存在显著相关性。多变量线性分析显示，年龄（β = -0.295，P < 0.001）、BMI（β = 0.464，P < 0.001）、RANKL（β = -0.105，P = 0.014）和OPG（β = 0.098，P = 0.029）是腰椎骨密度的独立决定因素（R² = 0.35）。年龄（β = -0.250，P < 0.001）、BMI（β = 0.486，P < 0.001）和RANKL（β = -0.110，P = 0.009）与股骨颈骨密度独立相关（R² = 0.36）。通过log转换后的hsCRP四分位数进行年龄和BMI校正分析，未发现与骨密度、骨转换生化标志物血清水平、RANKL或OPG之间存在关联。