利用高效液相色谱法对诺卡氏菌属CS682培养物中的那尔菌素进行定量分析。
Quantitative analysis of nargenicin in Nocardia sp. CS682 culture by high performance liquid chromatography.
作者信息
Cho Seung Sik, Sohng Jae Kyung, Lee Hyo Jeong, Park Sung Ju, Simkhada Jaya Ram, Yoo Jin Cheol
机构信息
Department of Pharmacy, Chosun University, Gwang Ju, 501-759, Korea.
出版信息
Arch Pharm Res. 2009 Mar;32(3):335-40. doi: 10.1007/s12272-009-1304-0. Epub 2009 Apr 23.
A simple and sensitive HPLC method was developed for the quantitative analysis of nargenicin in the culture broth of Nocardia sp. CS682, isolated from Korean soil. Nargenicin, an unusual macrolide antibiotic with potent anti-MRSA (methicilin-resistant Staphylococcus aureus) activity, was extracted from the culture broth with ethylacetate and then analyzed by HPLC using solvent A (0.5 M dipotassium phosphate pH 2.5-water: acetonitrile = 80:20, v/v) and solvent B (100% acetonitrile), at 215 nm. The calibration curve of nargenicin was linear with correlation coefficient greater than 0.99. This method provides precise analysis of the nargenicin amount present in the cell free culture medium, and may be applicable to analyze other bacterial cultures containing similar compounds.
开发了一种简单灵敏的高效液相色谱法，用于定量分析从韩国土壤中分离出的诺卡氏菌属CS682菌株培养液中的纳格菌素。纳格菌素是一种具有强效抗耐甲氧西林金黄色葡萄球菌（MRSA）活性的特殊大环内酯类抗生素，用乙酸乙酯从培养液中提取，然后通过高效液相色谱法在215nm波长下使用溶剂A（0.5M磷酸氢二钾，pH2.5 - 水：乙腈 = 80:20，v/v）和溶剂B（100%乙腈）进行分析。纳格菌素的校准曲线呈线性，相关系数大于0.99。该方法能精确分析无细胞培养基中纳格菌素的含量，可能适用于分析含有类似化合物的其他细菌培养物。
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