Sayari A, Gargouri Y
Laboratoire de Biochimie et de Génie Enzymatique des Lipases ENIS, BPW 3038-Sfax, Tunisie.
Arch Inst Pasteur Tunis. 2006;83(1-4):63-9.
The study of triacylglycerols synthesis using 1,3-regiospecific immobilized Rhizomucor miehei lipase (lipozyme) or non-regiospecific immobilized Candida antarctica lipase (novozyme) as biocatalyst was carried out in pure substrates conditions. Our results show that long-chain triacylglycerols were synthesized from glycerol and free fatty acids at a higher rate than medium-chain triacylglycerols which were themselves synthesized at a higher rate than short-chain ones. Furthermore, it is clearly shown that linoleic acid is more slowly esterifled than oleic acid which is itself more slowly esterified than octadecanoic acid. The higher the number of unsaturation, the lower the rate of synthesis and the final yield. On the other hand, the final yield of synthesis is comparable when using specific or non-specific lipase, as biocatalyst.
在纯底物条件下，开展了以1,3-区域特异性固定化米黑根毛霉脂肪酶（脂肪酶）或非区域特异性固定化南极假丝酵母脂肪酶（诺维信）作为生物催化剂来合成三酰甘油的研究。我们的结果表明，由甘油和游离脂肪酸合成长链三酰甘油的速率高于中链三酰甘油，而中链三酰甘油自身的合成速率又高于短链三酰甘油。此外，还清楚地表明，亚油酸的酯化比油酸慢，而油酸本身的酯化又比十八烷酸慢。不饱和度越高，合成速率和最终产率越低。另一方面，使用特异性或非特异性脂肪酶作为生物催化剂时，合成的最终产率相当。
