利用金枪鱼油选择性水解的副产物，在 sn-1(3)位一锅酶法合成富含二十二碳六烯酸的三酰基甘油。
One-pot enzymatic synthesis of docosahexaenoic acid-rich triacylglycerols at the sn-1(3) position using by-product from selective hydrolysis of tuna oil.
机构信息
Biomaterials and Commodity Chemicals Research Division, Osaka Municipal Technical Research Institute, Osaka 536-8553, Japan.
出版信息
N Biotechnol. 2011 Jan 31;28(1):7-13. doi: 10.1016/j.nbt.2010.07.021. Epub 2010 Aug 10.
Docosahexaenoic acid (DHA)-rich oil has been industrially produced by selective hydrolysis of tuna oil with a lipase that acts weakly on DHA. The free fatty acids (FFAs) generated in this process as by-products contain a high DHA concentration (46wt%) but are treated as industrial waste. This study attempted to reuse these by-product FFAs using a one-pot process, and succeeded in producing triacylglycerols (TAGs) through the esterification of the by-product FFAs with glycerol using immobilized Rhizomucor miehei lipase. Regiospecific analysis of the resulting TAGs showed that the content of DHA at the sn-1(3) position (51.7mol%) was higher than the content of DHA at the sn-2 position (17.3mol%). The DHA distribution in TAGs synthesized in this study was similar to the DHA distribution in TAGs from seal oil.
二十二碳六烯酸（DHA）含量丰富的油已通过用对 DHA 作用较弱的脂肪酶选择性水解金枪鱼油而在工业上生产。此过程中生成的作为副产物的游离脂肪酸（FFA）含有高浓度的 DHA（46wt％），但被视为工业废料。本研究试图使用一锅法再利用这些副产物 FFA，并成功地使用固定化米黑根毛霉脂肪酶通过副产物 FFA 与甘油的酯化反应生产了三酰基甘油（TAG）。对所得 TAG 的位置特异性分析表明，sn-1（3）位（51.7mol％）的 DHA 含量高于 sn-2 位（17.3mol％）的 DHA 含量。在本研究中合成的 TAG 中的 DHA 分布与海豹油中 TAG 的 DHA 分布相似。
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