Pan Yufeng, Zhou Yanqiong, Guo Chao, Gong Haiyun, Gong Zhefeng, Liu Li
State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, People's Republic of China.
Learn Mem. 2009 Apr 23;16(5):289-95. doi: 10.1101/lm.1331809. Print 2009 May.
The central complex is a prominent structure in the Drosophila brain. Visual learning experiments in the flight simulator, with flies with genetically altered brains, revealed that two groups of horizontal neurons in one of its substructures, the fan-shaped body, were required for Drosophila visual pattern memory. However, little is known about the role of other components of the central complex for visual pattern memory. Here we show that a small set of neurons in the ellipsoid body, which is another substructure of the central complex and connected to the fan-shaped body, is also required for visual pattern memory. Localized expression of rutabaga adenylyl cyclase in either the fan-shaped body or the ellipsoid body is sufficient to rescue the memory defect of the rut(2080) mutant. We then performed RNA interference of rutabaga in either structure and found that they both were required for visual pattern memory. Additionally, we tested the above rescued flies under several visual pattern parameters, such as size, contour orientation, and vertical compactness, and revealed differential roles of the fan-shaped body and the ellipsoid body for visual pattern memory. Our study defines a complex neural circuit in the central complex for Drosophila visual pattern memory.
中央复合体是果蝇大脑中的一个显著结构。在飞行模拟器中对基因改造大脑的果蝇进行视觉学习实验发现，其一个子结构扇形体中的两组水平神经元是果蝇视觉模式记忆所必需的。然而，对于中央复合体的其他组成部分在视觉模式记忆中的作用知之甚少。在这里，我们表明，中央复合体的另一个子结构椭圆体中一小部分神经元，且该部分神经元与扇形体相连，对于视觉模式记忆也是必需的。在扇形体或椭圆体中局部表达rutabaga腺苷酸环化酶足以挽救rut(2080)突变体的记忆缺陷。然后我们在这两种结构中对rutabaga进行RNA干扰，发现它们对于视觉模式记忆都是必需的。此外，我们在几个视觉模式参数下测试了上述挽救的果蝇，如大小、轮廓方向和垂直紧凑性，并揭示了扇形体和椭圆体在视觉模式记忆中的不同作用。我们的研究定义了中央复合体中用于果蝇视觉模式记忆的复杂神经回路。
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