使用抗恰加斯利什曼原虫超免疫血清的免疫过氧化物酶技术在培养确诊的美洲皮肤利什曼病诊断中的应用
Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis.
作者信息
Quintella Leonardo P, Cuzzi Tullia, Madeira Maria de F, Okamoto Thais, Schubach Armando de O
机构信息
Departamento de Patologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ. Brasil.
出版信息
Rev Inst Med Trop Sao Paulo. 2009 Mar-Apr;51(2):83-6. doi: 10.1590/s0036-46652009000200005.
The present study reports the production of the rabbit anti-Leishmania (L.) chagasi hyperimmune serum, the standardization of the immunohistochemistry (IHC) technique and the evaluation of its employment in cutaneous leishmaniasis (CL) lesions diagnosed by Leishmania sp. culture isolation. Thirty fragments of active CL lesions were examined as well as 10 fragments of cutaneous mycosis lesions as control group. IHC proved more sensitive in detecting amastigotes than conventional hematoxylin-eosin (HE) stained slides: the former was positive in 24 (80%) biopsies whereas the latter, in 16 (53%) (p = 0.028). The reaction stained different fungus species causing cutaneous mycosis. Besides, positive reaction was noticed in mononuclear and endothelial cells. Nevertheless, this finding was present in the control group biopsies. It is concluded that IHC showed good sensitivity in detecting amastigotes.
本研究报告了兔抗恰加斯利什曼原虫超免疫血清的制备、免疫组织化学（IHC）技术的标准化及其在利什曼原虫培养分离诊断的皮肤利什曼病（CL）病变中的应用评估。检查了30个活动性CL病变的切片以及10个皮肤真菌病病变的切片作为对照组。免疫组织化学在检测无鞭毛体方面比传统苏木精-伊红（HE）染色切片更敏感：前者在24例（80%）活检中呈阳性，而后者在16例（53%）中呈阳性（p = 0.028）。该反应对引起皮肤真菌病的不同真菌种类进行了染色。此外，在单核细胞和内皮细胞中观察到阳性反应。然而，这一发现也存在于对照组活检中。得出的结论是，免疫组织化学在检测无鞭毛体方面显示出良好的敏感性。
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