两种不同的归巢途径，涉及整合素 β7 和 E-选择素，显著影响沙眼衣原体感染后 CD4 细胞向生殖道的迁移。
Two different homing pathways involving integrin β7 and E-selectin significantly influence trafficking of CD4 cells to the genital tract following Chlamydia muridarum infection.
机构信息
Department of Pathology & Laboratory Medicine, Geffen School of Medicine, University of California, Los Angeles, CA 90095-1732, USA.
出版信息
Am J Reprod Immunol. 2009 Jun;61(6):438-45. doi: 10.1111/j.1600-0897.2009.00704.x. Epub 2009 Apr 22.
Abstract
PROBLEM
Chlamydia trachomatis causes STI and reproductive dysfunction worldwide which is not preventable with antibiotics. Identifying a population of endocervical T cells to target in vaccine development would enhance efficacy.
METHOD OF STUDY
Trafficking of murine CD4+ lymphocytes to Chlamydia muridarum infected genital tract (GT) tissue in vivo was measured using adoptive transfer studies of fluorescent CD4+ T cells from integrin β7-/- mice or mice which lack E-selectin on endothelial cells.
RESULTS
Murine in vivo migration studies showed that lack of α4β7 or E-selectin significantly reduced trafficking of CD4 T cells to the GT of mice infected with C. muridarum.
CONCLUSION
CD4+ T cells use at least two different adhesive mechanisms involving an integrin of the mucosal homing pathway and selectin pathway to accumulate in the GT during C. muridarum infection.
摘要
问题
沙眼衣原体可导致性传播感染和生殖功能障碍，而抗生素对此无法预防。如果能识别出可作为疫苗开发靶点的宫颈内 T 细胞群，将能提高疫苗的效果。
研究方法
通过将整合素β7-/- 小鼠或缺乏内皮细胞 E-选择素的小鼠的荧光 CD4+T 细胞过继转移至感染鼠型沙眼衣原体（Chlamydia muridarum）的生殖道组织，体内测量了鼠源 CD4+淋巴细胞向受染生殖道组织的转运情况。
结果
体内迁移研究显示，缺乏 α4β7 或 E-选择素会显著减少 CD4 T 细胞向感染鼠型沙眼衣原体的小鼠生殖道的转运。
结论
在鼠型沙眼衣原体感染期间，CD4+T 细胞至少使用两种不同的黏附机制，包括黏膜归巢途径的整合素和选择素途径，从而在生殖道内聚集。
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本文引用的文献
1
Bridging the knowledge gaps in vaccine design.
Nat Biotechnol. 2007 Dec;25(12):1361-6. doi: 10.1038/nbt1207-1361.
2
Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)(+) CD4(+) T cells.
Immunol Lett. 2007 Feb 15;108(2):179-82. doi: 10.1016/j.imlet.2006.12.002. Epub 2007 Jan 3.
3
Mucosal vaccines: the promise and the challenge.
Nat Rev Immunol. 2006 Feb;6(2):148-58. doi: 10.1038/nri1777.
4
Immunology of Chlamydia infection: implications for a Chlamydia trachomatis vaccine.
Nat Rev Immunol. 2005 Feb;5(2):149-61. doi: 10.1038/nri1551.
5
Reciprocal and dynamic control of CD8 T cell homing by dendritic cells from skin- and gut-associated lymphoid tissues.
J Exp Med. 2005 Jan 17;201(2):303-16. doi: 10.1084/jem.20041645. Epub 2005 Jan 10.
6
The infecting dose of Chlamydia muridarum modulates the innate immune response and ascending infection.
Infect Immun. 2004 Nov;72(11):6330-40. doi: 10.1128/IAI.72.11.6330-6340.2004.
7
P-selectin and P-selectin glycoprotein ligand 1 are major determinants for Th1 cell recruitment to nonlymphoid effector sites in the intestinal lamina propria.
J Exp Med. 2003 Aug 4;198(3):369-77. doi: 10.1084/jem.20020691. Epub 2003 Jul 28.
8
Selective imprinting of gut-homing T cells by Peyer's patch dendritic cells.
Nature. 2003 Jul 3;424(6944):88-93. doi: 10.1038/nature01726.
9
A Chlamydia trachomatis-specific Th2 clone does not provide protection against a genital infection and displays reduced trafficking to the infected genital mucosa.
Infect Immun. 2002 Sep;70(9):5132-9. doi: 10.1128/IAI.70.9.5132-5139.2002.
10
E- and P-selectins are essential for the development of cockroach allergen-induced airway responses.
J Immunol. 2002 Aug 15;169(4):2120-5. doi: 10.4049/jimmunol.169.4.2120.
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