Maxion Heather K, Liu Wei, Chang Mi-Hyang, Kelly Kathleen A
David Geffen School of Medicine at UCLA, Department of Pathology & Laboratory Medicine, Los Angeles, CA 90095-1732, USA.
Infect Immun. 2004 Nov;72(11):6330-40. doi: 10.1128/IAI.72.11.6330-6340.2004.
Murine vaginal infection with the obligate intracellular bacterium Chlamydia muridarum is commonly used as a model for ascending Chlamydia infections of the human female genital tract. Gamma interferon-producing Th1 cells, in concert with other mononuclear infiltrates, primarily mediate antichlamydial immunity. However, many factors modify this response, including the bacterial load. To investigate the manner in which the inoculating dose of C. muridarum modulates a genital infection, we measured innate and adaptive cell numbers, CD4+ lymphocyte cytokine profile, chemokine expression, course of infection, and pathological sequelae in genital tracts of BALB/c mice infected with doses of C. muridarum ranging from 10(4) to 10(7) inclusion-forming units. We found that the influx of both innate and adaptive immune cells responded similarly in the lower genital tract (cervical-vaginal tissues) and upper genital tract (oviduct tissues) to increasing inoculating doses. However, cells expressing the innate markers Gr-1 and CD11c were affected to a greater degree by increasing dose than lymphocytes of the adaptive immune response (Th1, CD4+, CD8+, CD19+), resulting in a change in the balance of innate and adaptive cell numbers to favor innate cells at higher infecting doses. Surprisingly, we detected greater numbers of viable chlamydiae in the oviducts at lower inoculating doses, and the number of organisms appeared to directly correlate with hydrosalpinx formation after both primary infection and repeat infection. Taken together, these data suggest that innate immune cells contribute to control of ascending infection.
将专性胞内细菌鼠衣原体接种到小鼠阴道中，常用于模拟人类女性生殖道衣原体上行感染的模型。产生γ干扰素的Th1细胞与其他单核浸润细胞协同作用，主要介导抗衣原体免疫。然而，许多因素会改变这种反应，包括细菌载量。为了研究鼠衣原体接种剂量调节生殖器感染的方式，我们测量了感染剂量范围为10⁴至10⁷包涵体形成单位的鼠衣原体的BALB/c小鼠生殖道中的固有细胞和适应性细胞数量、CD4⁺淋巴细胞细胞因子谱、趋化因子表达、感染过程和病理后遗症。我们发现，固有免疫细胞和适应性免疫细胞的流入在较低生殖道（宫颈-阴道组织）和较高生殖道（输卵管组织）中对增加的接种剂量反应相似。然而，与适应性免疫反应的淋巴细胞（Th1、CD4⁺、CD8⁺、CD19⁺）相比，表达固有标志物Gr-1和CD11c的细胞受剂量增加的影响更大，导致固有细胞和适应性细胞数量的平衡发生变化，在较高感染剂量下有利于固有细胞。令人惊讶的是，我们在较低接种剂量的输卵管中检测到更多存活的衣原体，并且在初次感染和重复感染后，生物体数量似乎与输卵管积水的形成直接相关。综上所述，这些数据表明固有免疫细胞有助于控制上行感染。
