Neely A N, Childress C M, Maley M P, Holder I A
Shriners Burns Institute, Cincinnati, OH 45219.
J Burn Care Rehabil. 1991 Jul-Aug;12(4):294-9. doi: 10.1097/00004630-199107000-00003.
The progression of autograft colonization to infection presents significant problems in the care of grafted burn wounds. To determine whether graft colonization arises from self-contamination or from some exogenous source, we characterized pregraft and graft Candida albicans isolates through serotyping and biotyping (Leicester system) and Pseudomonas aeruginosa isolates through serotyping (Japan Pseudomonas aeruginosa Society system). In 21 of 24 patients, the serotype/biotype of the graft isolate was the same as the serotype/biotype of a C. albicans strain previously isolated from the same patient. Fifteen of 16 P. aeruginosa graft contaminants also showed the same serotype as that in a pregraft isolate. Therefore, whereas it has long been surmised that the grafts of patients with burns are self-colonized, this study provides definite data showing that most C. albicans and P. aeruginosa autograft isolates are indeed self-contaminants.
自体移植皮片定植发展为感染给烧伤创面移植皮片的护理带来了重大问题。为了确定移植皮片定植是源于自身污染还是某些外源性来源，我们通过血清分型和生物分型（莱斯特系统）对移植前和移植后的白色念珠菌分离株进行了特征分析，并通过血清分型（日本铜绿假单胞菌协会系统）对铜绿假单胞菌分离株进行了特征分析。在24例患者中的21例中，移植分离株的血清型/生物型与先前从同一患者分离出的白色念珠菌菌株的血清型/生物型相同。16例铜绿假单胞菌移植污染物中的15例也显示出与移植前分离株相同的血清型。因此，尽管长期以来一直推测烧伤患者的移植皮片是自身定植的，但本研究提供了确切数据表明，大多数白色念珠菌和铜绿假单胞菌自体移植分离株确实是自身污染物。
