Abdeev Rustam M, Abdeeva Inna A, Bruskin Sergey S, Musiychuk Konstantin A, Goldenkova-Pavlova Irina V, Piruzian Eleonora S
Center for Theoretical Problems of Physico-Chemical Pharmacology RAS, Moscow, Russia.
Gene. 2009 May 1;436(1-2):81-9. doi: 10.1016/j.gene.2009.01.021. Epub 2009 Feb 12.
A new strategy for creating experimental models for functional genomics has been proposed. It is based on the expression in transgenic plants of genes from thermophilic bacteria encoding functional analogues of plant proteins with high specific activity and thermal stability. We have validated this strategy by comparing physiological, biochemical and molecular properties of control tobacco plants and transgenic plants expressing genes of beta-glucanases with different substrate specificity. We demonstrate that the expression of bacterial beta-1,3-1,4-glucanase gene exerts no significant influence on tobacco plant metabolism, while the expression of bacterial beta-1,3-glucanase affects plant metabolism only at early stages of growth and development. By contrast, the expression of bacterial beta-1,4-glucanase has a significant effect on transgenic tobacco plant metabolism, namely, it affects plant morphology, the thickness of the primary cell wall, phytohormonal status, and the relative sugar content. We propose a hypothesis of beta-glucanase action as an important factor of genetic regulation of metabolic processes in plants.
一种用于创建功能基因组学实验模型的新策略已被提出。它基于在转基因植物中表达来自嗜热细菌的基因，这些基因编码具有高比活性和热稳定性的植物蛋白功能类似物。我们通过比较对照烟草植株和表达具有不同底物特异性的β-葡聚糖酶基因的转基因植株的生理、生化和分子特性，验证了这一策略。我们证明，细菌β-1,3-1,4-葡聚糖酶基因的表达对烟草植株代谢没有显著影响，而细菌β-1,3-葡聚糖酶的表达仅在生长和发育的早期阶段影响植物代谢。相比之下，细菌β-1,4-葡聚糖酶的表达对转基因烟草植株代谢有显著影响，即它影响植物形态、初生细胞壁厚度、植物激素状态和相对糖含量。我们提出了β-葡聚糖酶作为植物代谢过程遗传调控重要因素的作用假说。
