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来自大肠杆菌的曲霉属CJ22 - 326外切-β-D-氨基葡萄糖苷酶的表达、纯化及特性分析

Expression, purification, and characterization of exo-beta-D-glucosaminidase of Aspergillus sp. CJ22-326 from Escherichia coli.

作者信息

Li Songlin, Wang Chen, Xia Wenshui

机构信息

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China.

出版信息

Carbohydr Res. 2009 May 26;344(8):1046-9. doi: 10.1016/j.carres.2009.02.026. Epub 2009 Mar 4.

DOI:10.1016/j.carres.2009.02.026
PMID:19393602
Abstract

An exo-beta-D-glucosaminidase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an exo-chitosanase activity in a viscosimetric assay and TLC analysis. This is the first report on cloning of a gene encoding an Aspergillus sp. exo-beta-D-glucosaminidase.

摘要

从曲霉属菌株CJ22 - 326中克隆出一个外切-β-D-氨基葡萄糖苷酶基因，并在大肠杆菌中进行表达。经纯化的蛋白在粘度测定和薄层色谱分析中显示出外切壳聚糖酶活性。这是关于曲霉属菌株外切-β-D-氨基葡萄糖苷酶编码基因克隆的首次报道。

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