Kowalczynska H M, Kaminski J
Department of Biophysics and Biomathematics, Medical Center of Postgraduate Education, Warszawa, Poland.
J Cell Sci. 1991 Jul;99 ( Pt 3):587-93. doi: 10.1242/jcs.99.3.587.
The static adhesion of living L1210 cells (in serum-containing medium) to the surface of (styrene/methylmethacrylate) copolymers differing in styrene content (from 5% to 50% of styrene units) was investigated. The examination of wettability of the copolymer surfaces showed that the contact angle of water on the hydrophobic surfaces is an increasing linear function of styrene content in the copolymer. Cell adhesion to the unwettable surfaces is low (within 2-4%). A novel method of modification of the styrene copolymer surfaces was used to render these surfaces suitable for cell attachment. The modification consists of sulfonation of the surfaces with sulfur trioxide at the gas/solid interface. The contact angle of sulfonated copolymer surfaces is a decreasing linear function of styrene content in the copolymer. The contact angle decreases due to the increased number of highly hydrophilic sulfonic groups bonded to styrene. By acetylation of the sulfonated surfaces it was shown that cell adhesion to acetylated surfaces is not diminished and is at the same level as cell adhesion to sulfonated copolymer surfaces. Thus, it can be concluded that sulfonation of copolymer surfaces does not form hydroxyl groups. Cell adhesion to substrata of high wettability stabilizes after 30s. The relative number of cells adhering to the sulfonated copolymer surfaces is a decreasing linear function of the contact angle. For the copolymer surfaces containing 50% of styrene units the contact angle decreases sevenfold, due to sulfonation, and the number of adhering cells increases 40-fold. The results obtained show that for cell-substratum adhesive interaction the presence of sulfonic groups at the substratum surface is important.
研究了活的L1210细胞（在含血清培养基中）对苯乙烯含量不同（苯乙烯单元占5%至50%）的（苯乙烯/甲基丙烯酸甲酯）共聚物表面的静态粘附。对共聚物表面润湿性的检测表明，水在疏水表面上的接触角是共聚物中苯乙烯含量的线性递增函数。细胞对不可润湿表面的粘附力较低（在2%至4%之间）。采用一种新型的苯乙烯共聚物表面改性方法，使这些表面适合细胞附着。改性包括在气/固界面用三氧化硫对表面进行磺化。磺化共聚物表面的接触角是共聚物中苯乙烯含量的线性递减函数。由于与苯乙烯键合的高亲水性磺酸基团数量增加，接触角减小。通过对磺化表面进行乙酰化处理表明，细胞对乙酰化表面的粘附力没有降低，与细胞对磺化共聚物表面的粘附力处于同一水平。因此，可以得出结论，共聚物表面的磺化不会形成羟基。细胞对高润湿性基质的粘附在30秒后稳定下来。粘附在磺化共聚物表面的细胞相对数量是接触角的线性递减函数。对于含有50%苯乙烯单元的共聚物表面，由于磺化，接触角减小了七倍，粘附细胞的数量增加了40倍。所得结果表明，对于细胞-基质粘附相互作用，基质表面存在磺酸基团很重要。
