Linde S, Røder M E, Hartling S G, Binder C, Welinder B S
Hagedorn Research Laboratory, Gentofte, Denmark.
J Chromatogr. 1991 Jul 12;548(1-2):371-80. doi: 10.1016/s0021-9673(01)88620-9.
Two reversed-phase high-performance liquid chromatographic (RP-HPLC) systems were developed for the separation of human insulin, proinsulin and the major proinsulin intermediates. The individual components were quantified using two enzyme-linked immunosorbent assays for insulin and proinsulin immunore-active material (PIM) after (passive) evaporation of the organic modifier. Serum samples from normal subjects and patients with non-insulin-dependent diabetes mellitus were immunopurified and analysed in one of the RP-HPLC systems. The proportion of PIM relative to insulin immunoreactive material was higher in the diabetic patient compared with that in the normal subject. In both, PIM was heterogeneous, consisting of intact proinsulin and des-proinsulin intermediates.
开发了两种反相高效液相色谱（RP-HPLC）系统，用于分离人胰岛素、胰岛素原和主要的胰岛素原中间体。在（被动）蒸发有机改性剂后，使用两种酶联免疫吸附测定法对胰岛素和胰岛素原免疫反应性物质（PIM）进行定量分析，以确定各个成分的含量。对正常受试者和非胰岛素依赖型糖尿病患者的血清样本进行免疫纯化，并在其中一种RP-HPLC系统中进行分析。与正常受试者相比，糖尿病患者中PIM相对于胰岛素免疫反应性物质的比例更高。在两者中，PIM都是异质的，由完整的胰岛素原和去胰岛素原中间体组成。
