Vavassori Stefano, Covey Lori R
Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.
RNA Biol. 2009 Jul-Aug;6(3):259-65. doi: 10.4161/rna.6.3.8581. Epub 2009 Jul 29.
The control of mRNA decay is emerging as an important control point and a major contributor to gene expression in both immune and non-immune cells. The identification of protein factors and cis-acting elements responsible for transcript degradation has illuminated a comprehensive picture of precisely orchestrated events required to both regulate and establish the decay process. One gene that is highly regulated at the post-transcriptional level is CD40 ligand (CD154 or CD40L). CD154 on CD4(+) T cells is tightly controlled by an interacting network of transcriptional and post-transcriptional processes that result in precise surface levels of protein throughout an extended time course of antigen stimulation. The activation-induced stabilization of the CD154 transcript by a polypyrimidine tract-binding protein (PTB)-complex is a key event that corresponds to the temporal expression of CD154. In this review, we discuss known and potential roles of major mRNA decay pathways in lymphocytes and focus on the unique post-transcriptional mechanisms leading to CD154 expression by activated CD4(+) T cells.
mRNA 降解的调控正成为一个重要的控制点，并且是免疫细胞和非免疫细胞中基因表达的主要贡献因素。负责转录本降解的蛋白质因子和顺式作用元件的鉴定，揭示了精确调控和建立降解过程所需的精心编排事件的全貌。一个在转录后水平受到高度调控的基因是 CD40 配体（CD154 或 CD40L）。CD4(+) T 细胞上的 CD154 受到转录和转录后过程相互作用网络的严格控制，这导致在抗原刺激的延长时间过程中蛋白质的精确表面水平。多嘧啶序列结合蛋白（PTB）复合物对 CD154 转录本的激活诱导稳定作用是一个关键事件，它与 CD154 的时间表达相对应。在这篇综述中，我们讨论了主要 mRNA 降解途径在淋巴细胞中的已知和潜在作用，并重点关注导致活化的 CD4(+) T 细胞表达 CD154 的独特转录后机制。
