Heitz Benjamin A, Xu Juhua, Hall Henry K, Aspinwall Craig A, Saavedra S Scott
Department of Chemistry, University of Arizona, 1306 East University Boulevard, Tucson, Arizona 85721, USA.
J Am Chem Soc. 2009 May 20;131(19):6662-3. doi: 10.1021/ja901442t.
Black lipid membranes (BLMs) are widely used for recording the activity of incorporated ion channel proteins. However, BLMs are inherently unstable structures that typically rupture within a few hours after formation. Here, stabilized BLMs were formed using the polymerizable lipid bis-dienoyl phosphatidylcholine (bis-DenPC) on glass pipettes of approximately 10 microm (I.D.). After polymerization, these BLMs maintained steady conductance values for several weeks, as compared to a few hours for unpolymerized membranes. The activity of an ion channel, alpha-hemolysin, incorporated into bis-DenPC BLMs prior to polymerization, was maintained for 1 week after BLM formation and polymerization. These lifetimes are a substantial improvement over those achievable with conventional BLM technologies. Polymerized BLMs containing functional ion channels may represent an enabling technology for development of robust biosensors and drug screening devices.
黑色脂质膜（BLMs）被广泛用于记录所掺入离子通道蛋白的活性。然而，BLMs本质上是不稳定的结构，通常在形成后几小时内就会破裂。在这里，使用可聚合脂质双二烯酰磷脂酰胆碱（bis-DenPC）在内径约为10微米的玻璃移液管上形成了稳定的BLMs。聚合后，与未聚合的膜仅能维持几小时相比，这些BLMs能在几周内保持稳定的电导值。在聚合前掺入bis-DenPC BLMs的离子通道α-溶血素的活性，在BLM形成和聚合后可维持1周。这些寿命比传统BLM技术所能达到的有了显著提高。含有功能性离子通道的聚合BLMs可能代表了一种用于开发强大生物传感器和药物筛选设备的使能技术。
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