Garzón G A, Riedl K M, Schwartz S J
Dept. de Química, Univ. Nacional de Colombia, AA 14490 Bogotá, Colombia.
J Food Sci. 2009 Apr;74(3):C227-32. doi: 10.1111/j.1750-3841.2009.01092.x.
Anthocyanins, total phenolic content, ascorbic acid content, and the antioxidant activity were determined in extracts of Andes berry fruit (Rubus glaucus Benth). Anthocyanis (ACNs) were isolated and characterized by means of high-performance liquid chromatography (HPLC) with photodiode array detection and electro spray ionization/mass spectrometry (PDA-ESI/MS/MS) analysis. The anthocyanin (ACN) content was 45 mg/100 g FW. The isolated anthocyanins were characterized as cyanidin 3-sambubioside, cyanidin 3-glucoside, cyanidin 3-xylorutinoside, cyanidin 3-rutinoside, pelargonidin 3-glucoside, and pelargonidin 3-rutinoside. The ascorbic acid content was 10.1 mg/100 g FW. The total phenolic content as determined by the Folin-Ciocalteau method was 294 mg GAE/100 g FW while the antioxidant activity as measured by ABTS(.) (+) radical scavenging capacity and ferric reducing antioxidant power (FRAP) was 2.01 and 4.50 mmol TE/100 g FW or 8.22 mmoles ferric iron reduced/100 g FW, respectively. The high phenolic content and antioxidant capacity of Andes berry suggest that this fruit could be a rich source of natural pigments, nutraceuticals, and natural antioxidants.
测定了安第斯莓果实（智利黑莓）提取物中的花色苷、总酚含量、抗坏血酸含量和抗氧化活性。通过高效液相色谱（HPLC）结合光电二极管阵列检测和电喷雾电离/质谱（PDA-ESI/MS/MS）分析对花色苷进行分离和表征。花色苷含量为45 mg/100 g鲜重。分离得到的花色苷鉴定为矢车菊素3-桑布双糖苷、矢车菊素3-葡萄糖苷、矢车菊素3-木糖芦丁糖苷、矢车菊素3-芦丁糖苷、天竺葵素3-葡萄糖苷和天竺葵素3-芦丁糖苷。抗坏血酸含量为10.1 mg/100 g鲜重。用福林-酚法测定的总酚含量为294 mg没食子酸当量/100 g鲜重，而用ABTS(.)(+)自由基清除能力和铁还原抗氧化能力（FRAP）测定的抗氧化活性分别为2.01和4.50 mmol TE/100 g鲜重或8.22 mmol三价铁还原/100 g鲜重。安第斯莓较高的酚类含量和抗氧化能力表明，这种水果可能是天然色素、营养保健品和天然抗氧化剂的丰富来源。
