Albino A P, Nanus D M, Davis M L, McNutt N S
Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
J Cutan Pathol. 1991 Aug;18(4):273-8. doi: 10.1111/j.1600-0560.1991.tb01235.x.
A number of studies have failed to detect point mutations at codon 12 in the Ki-ras gene in melanocytic neoplasms. One recent study, however, has found a high percentage of Ki-ras codon 12 point mutations. In an effort to resolve this difference, the present study examined noncultured melanocytic lesions (i.e., 5 benign nevi, 10 dysplastic nevi, and 8 primary melanomas: 4 in situ and 4 invasive) for point mutations at codon 12 in the first exon of the Ki-ras proto-oncogene using polymerase chain reaction methodology with oligonucleotide hybridization and direct DNA sequencing. The results of this study indicates no detectable mutations in the 12th codon of the first exon of the Ki-ras gene in any premalignant or malignant melanocytic lesion examined.
多项研究未能在黑素细胞性肿瘤的Ki-ras基因第12密码子处检测到点突变。然而，最近的一项研究发现Ki-ras基因第12密码子点突变的比例很高。为了解决这一差异，本研究采用聚合酶链反应方法结合寡核苷酸杂交和直接DNA测序，检测了非培养的黑素细胞性病变（即5例良性痣、10例发育异常痣和8例原发性黑色素瘤：4例原位癌和4例浸润性癌）中Ki-ras原癌基因第一外显子第12密码子的点突变。本研究结果表明，在所检测的任何癌前或恶性黑素细胞性病变中，Ki-ras基因第一外显子第12密码子均未检测到突变。
