Sartori César Renato, Pelágio Fernanda Campos, Teixeira Simone Aparecida, Valentinuzzi Verónica Sandra, Nascimento André Luiz, Rogério Fábio, Muscará Marcelo Nicolás, Ferrari Elenice Aparecida de Moraes, Langone Francesco
Department of Physiology & Biophysics, Institute of Biology, State University of Campinas (UNICAMP), P.O. Box 6109, 13083-970, Campinas, SP, Brazil.
Behav Brain Res. 2009 Nov 5;203(2):165-72. doi: 10.1016/j.bbr.2009.04.022. Epub 2009 May 3.
Voluntary physical activity improves memory and learning ability in rodents, whereas status epilepticus has been associated with memory impairment. Physical activity and seizures have been associated with enhanced hippocampal expression of BDNF, indicating that this protein may have a dual role in epilepsy. The influence of voluntary physical activity on memory and BDNF expression has been poorly studied in experimental models of epilepsy. In this paper, we have investigated the effect of voluntary physical activity on memory and BDNF expression in mice with pilocarpine-induced epilepsy. Male Swiss mice were assigned to four experimental groups: pilocarpine sedentary (PS), pilocarpine runners (PRs), saline sedentary (SS) and saline runners (SRs). Two days after pilocarpine-induced status epilepticus, the affected mice (PR) and their running controls (SR) were housed with access to a running wheel for 28 days. After that, the spatial memory and the expression of the precursor and mature forms of hippocampal BDNF were assessed. PR mice performed better than PS mice in the water maze test. In addition, PR mice had a higher amount of mature BDNF (14kDa) relative to the total BDNF (14kDa+28kDa+32kDa forms) content when compared with PS mice. These results show that voluntary physical activity improved the spatial memory and increased the hippocampal content of mature BDNF of mice with pilocarpine-induced status epilepticus.
自愿性体育活动可改善啮齿动物的记忆力和学习能力，而癫痫持续状态则与记忆障碍有关。体育活动和癫痫发作都与海马体中脑源性神经营养因子（BDNF）表达增强有关，这表明该蛋白在癫痫中可能具有双重作用。在癫痫实验模型中，自愿性体育活动对记忆和BDNF表达的影响鲜有研究。在本文中，我们研究了自愿性体育活动对匹鲁卡品诱导癫痫小鼠的记忆和BDNF表达的影响。雄性瑞士小鼠被分为四个实验组：匹鲁卡品静息组（PS）、匹鲁卡品跑步组（PR）、生理盐水静息组（SS）和生理盐水跑步组（SR）。在匹鲁卡品诱导癫痫持续状态两天后，将受影响的小鼠（PR）及其跑步对照组（SR）饲养在可使用跑步轮的环境中28天。之后，评估空间记忆以及海马体BDNF前体和成熟形式的表达。在水迷宫测试中，PR组小鼠的表现优于PS组小鼠。此外，与PS组小鼠相比，PR组小鼠成熟BDNF（14kDa）相对于总BDNF（14kDa + 28kDa + 32kDa形式）的含量更高。这些结果表明，自愿性体育活动改善了匹鲁卡品诱导癫痫持续状态小鼠的空间记忆，并增加了其海马体中成熟BDNF的含量。
