de Almeida Alexandre Aparecido, Gomes da Silva Sérgio, Lopim Glauber Menezes, Vannucci Campos Diego, Fernandes Jansen, Cabral Francisco Romero, Arida Ricardo Mario
Departamento de Fisiologia, Universidade Federal de São Paulo, Rua Botucatu 862, Ed. Ciências Biomédicas, 5° andar. Vila Clementino, São Paulo, SP, 04023-900, Brazil.
Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
Neurochem Res. 2017 Apr;42(4):1230-1239. doi: 10.1007/s11064-016-2165-9. Epub 2017 Jan 11.
Epilepsy is a disease characterized by recurrent, unprovoked seizures. Cognitive impairment is an important comorbidity of chronic epilepsy. Human and animal model studies of epilepsy have shown that aerobic exercise induces beneficial structural and functional changes and reduces the number of seizures. However, little is yet understood about the effects of resistance exercise on epilepsy. We evaluated the effects of a resistance exercise program on the number of seizures, long-term memory and expression/activation of signaling proteins in rats with epilepsy. The number of seizures was quantified by video-monitoring and long-term memory was assessed by an inhibitory avoidance test. Using western blotting, multiplex and enzyme-linked immunosorbent assays, we determined the effects of a 4-week resistance exercise program on IGF-1 and BDNF levels and ERK, CREB, mTOR activation in the hippocampus of rats with epilepsy. Rats with epilepsy submitted to resistance exercise showed a decrease in the number of seizures compared to non-exercised epileptic rats. Memory deficits were attenuated by resistance exercise. Rats with epilepsy showed an increase in IGF-1 levels which were restored to control levels by resistance exercise. BDNF levels and ERK and mTOR activation were decreased in rats with epilepsy and resistance exercise restored these to control levels. In conclusion, resistance exercise reduced seizure occurrence and mitigated memory deficits in rats with epilepsy. These resistance exercise-induced beneficial effects can be related to changes in IGF-1 and BDNF levels and its signaling protein activation. Our findings indicate that the resistance exercise might be included as complementary therapeutic strategy for epilepsy treatment.
癫痫是一种以反复发作、无诱因惊厥为特征的疾病。认知障碍是慢性癫痫的一种重要合并症。癫痫的人类和动物模型研究表明，有氧运动可诱导有益的结构和功能变化，并减少惊厥发作次数。然而，关于抗阻运动对癫痫的影响，目前了解甚少。我们评估了一项抗阻运动计划对癫痫大鼠惊厥发作次数、长期记忆以及信号蛋白表达/激活的影响。通过视频监测对惊厥发作次数进行量化，并通过抑制性回避试验评估长期记忆。使用蛋白质免疫印迹法、多重免疫分析法和酶联免疫吸附测定法，我们确定了一项为期4周的抗阻运动计划对癫痫大鼠海马中IGF-1和BDNF水平以及ERK、CREB、mTOR激活的影响。与未进行运动的癫痫大鼠相比，进行抗阻运动的癫痫大鼠惊厥发作次数减少。抗阻运动减轻了记忆缺陷。癫痫大鼠的IGF-1水平升高，而抗阻运动使其恢复到对照水平。癫痫大鼠的BDNF水平以及ERK和mTOR激活降低，抗阻运动将这些指标恢复到对照水平。总之，抗阻运动减少了癫痫大鼠的惊厥发作，并减轻了记忆缺陷。这些抗阻运动诱导的有益效果可能与IGF-1和BDNF水平及其信号蛋白激活的变化有关。我们的研究结果表明，抗阻运动可能作为癫痫治疗的辅助治疗策略。
