Li Wing-Yan, Chan Shun-Wan, Guo De-Jian, Chung Mei-Kuen, Leung Tin-Yan, Yu Peter Hoi-Fu
Open Laboratory of Chirotechnology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, China.
J Ethnopharmacol. 2009 Jul 15;124(2):251-6. doi: 10.1016/j.jep.2009.04.030. Epub 2009 May 3.
Rheum officinale Baill. (Da Huang) is one of the herbs commonly used in traditional Chinese medicine formulae against cancer. The traditional decoction is similar to the water extract used in the present study.
The water extract of Da Huang was investigated to see if it possesses anticancer effects through apoptotic pathways.
Human lung adenocarcinoma A549 and human breast cancer MCF-7 cell lines were treated with different concentrations of Da Huang water extract at different time intervals. Growth inhibition was detected by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] and colony formation assays; apoptosis was detected by cell morphologic analysis, DNA fragmentation analysis and COMET assay.
Da Huang water extract was found to have significant growth inhibitory effects on both A549 and MCF-7 cell lines with IC(50) values 620+/-12.7 and 515+/-10.1 microg/ml, respectively. Growth inhibitory effects were dose- and time-dependent. A significant decrease in cell number, DNA fragmentation and single DNA strand breakages were observed in the Da Huang water extract treated A549 and MCF-7 cells.
This suggests that the water extract of Da Huang exerts potential anticancer activity through growth inhibition and apoptosis on MCF-7 and A549 cells lines.
大黄是常用于抗癌中药配方的草药之一。传统汤剂与本研究中使用的水提取物相似。
研究大黄水提取物是否通过凋亡途径具有抗癌作用。
用人肺腺癌A549和人乳腺癌MCF-7细胞系，在不同时间间隔用不同浓度的大黄水提取物处理。通过MTS [3-(4,5-二甲基噻唑-2-基)-5-(3-羧基甲氧基苯基)-2-(4-磺基苯基)-2H-四唑] 和集落形成试验检测生长抑制；通过细胞形态分析、DNA片段化分析和彗星试验检测凋亡。
发现大黄水提取物对A549和MCF-7细胞系均有显著的生长抑制作用，IC(50)值分别为620±12.7和515±10.1微克/毫升。生长抑制作用呈剂量和时间依赖性。在大黄水提取物处理的A549和MCF-7细胞中观察到细胞数量、DNA片段化和单链DNA断裂显著减少。
这表明大黄水提取物通过对MCF-7和A549细胞系的生长抑制和凋亡发挥潜在的抗癌活性。
