Coutlée François, Trottier Helen, Gagnon Simon, Koushik Anita, Richardson Harriet, Roger Michel, Ferenczy Alex S, Franco Eduardo L
Département de Microbiologie-Infectiologie et Centre de Recherche, Centre Hospitalier de l'Université de Montréal (CHUM), Université de Montréal, Québec, Canada.
J Clin Virol. 2009 Jun;45(2):96-9. doi: 10.1016/j.jcv.2009.03.019. Epub 2009 Apr 26.
The association between human papillomavirus (HPV) viral load and high-grade squamous intraepithelial lesion (HSIL) of the uterine cervix has been demonstrated for high-risk HPV-16 but has not been investigated for low-risk HPV types.
To determine the association between the presence of low-grade SIL (LSIL) and viral load of low-risk HPV type 6.
107 HPV-6-positive cytobrush samples collected from 90 women (67 without SIL, 11 with LSIL, 5 with HSIL, 6 with SIL of unknown grade and 1 with a smear with LSIL and a normal colposcopy) were analyzed for their content of HPV-6 DNA.
HPV-6 E6 and E2 DNA loads were measured in the cytobrush samples with two real-time PCR assays. HPV-6 integration was confirmed with restriction-site PCR.
HPV-6 DNA in cervical samples ranged from 1.8 x 10(2) to 4.25 x 10(8) copies per microg of cellular DNA. HPV-6 E6 DNA loads were higher in women with LSIL, with or without co-infection with high-risk HPV types, than in women without SIL (p=0.03). HPV-6 loads greater than 8.76 x 10(6)copies per microgDNA, corresponding to the mean HPV-6 load measured in women with LSIL, were more frequently detected in these women with LSIL than in women without LSIL, controlling for age (odds ratio 13.5, 95% confidence interval 1.1-172.4). Although 10 samples generated HPV-6 E6/E2 ratios above 2, there was no evidence of integration of HPV-6 by restriction-site PCR.
Samples from women with LSIL contained higher HPV-6 loads than women without SIL. HPV-6 DNA was not integrated into cellular DNA in these samples.
人乳头瘤病毒（HPV）病毒载量与子宫颈高级别鳞状上皮内病变（HSIL）之间的关联已在高危型HPV - 16中得到证实，但尚未对低危型HPV进行研究。
确定低级别鳞状上皮内病变（LSIL）的存在与低危型HPV - 6病毒载量之间的关联。
从90名女性中收集了107份HPV - 6阳性的细胞刷样本（67名无鳞状上皮内病变，11名有LSIL，5名有HSIL，6名鳞状上皮内病变级别不明，1名涂片显示LSIL但阴道镜检查正常），分析其HPV - 6 DNA含量。
采用两种实时聚合酶链反应（PCR）测定细胞刷样本中的HPV - 6 E6和E2 DNA载量。通过限制性位点PCR确认HPV - 6整合情况。
宫颈样本中的HPV - 6 DNA含量为每微克细胞DNA 1.8×10²至4.25×10⁸拷贝。无论是否合并高危型HPV感染，有LSIL的女性中HPV - 6 E6 DNA载量均高于无鳞状上皮内病变的女性（p = 0.03）。在这些有LSIL的女性中，HPV - 6载量大于每微克DNA 8.76×10⁶拷贝（相当于有LSIL女性中测得的平均HPV - 6载量）的情况比无LSIL的女性更常见，校正年龄后（优势比13.5，95%置信区间1.1 - 172.4）。尽管有10个样本的HPV - 6 E6/E2比值高于2，但限制性位点PCR未发现HPV - 6整合的证据。
有LSIL的女性样本中HPV - 6载量高于无鳞状上皮内病变的女性。这些样本中HPV - 6 DNA未整合到细胞DNA中。
Zotero 插件