Sasagawa T, Basha W, Yamazaki H, Inoue M
School of Health Sciences, Faculty of Medicine, Kanazawa University, Ishikawa, Japan.
Cancer Epidemiol Biomarkers Prev. 2001 Jan;10(1):45-52.
To estimate the risk of human papillomavirus (HPV) infection for cervical malignancies, we conducted a case-control study in Japan. Abnormal cervical cell (366) and normal cell samples (1562) were tested for the presence of HPV DNA using a new PCR-based test (LCR-E7 PCR). When single HPV infections were considered, 26 different HPV types were identified in normal cervices and in low-grade squamous intraepithelial lesions (LSIL); whereas HPV-16, -18, -31, -33, -35, -45, -51, -52, -56, -58 and -67 were detected in high-grade squamous intraepithelial lesions (HSIL) and in squamous cell carcinoma (SCC) of the cervix, and HPV-16 and -18 were detected in cervical adenocarcinoma. HPV-6 and -11 were detected in condyloma acuminatum tissue. In HSIL and SCC, HPV-16 was the most prevalent type and HPV-51, -52, and -58 were the next most prevalent; whereas HPV-39, -59, and -68 were not detected. Analysis by odds ratio (OR) revealed that HPV-11, -39, -42, -44, -53, -59, -62, and -66 (HPV-66: OR,139; 95% confidence interval (CI) = 6.7-168) were associated with LSIL; HPV-16, -18, -31, -51, -52 and -58 (HPV-16: OR, 69; 95%CI = 36-131) were associated with SCC; and HPV-16 and -18 (OR, 94; 95% CI = 28-317) were associated with adenocarcinoma. Multiple HPV infection was associated with LSIL (OR, 24; 95%CI = 13-44), HSIL (OR, 16; 95%CI = 8.4-32), and SCC (OR, 8.3; 95%CI = 3.2-22), although the prevalence decreased with the grade of the lesions. All results suggest that HPV-6 and -11 are condyloma types, HPV-16, -18, -31, -51, -52, -58, and perhaps -33, -35, -45, -56, and -67, are the high-risk HPV types, and many other types are LSIL-associated types in Japan. HPV typing and detection of multiple HPV infections in clinical samples may be useful as surrogate markers for cervical cell abnormalities.
为评估人乳头瘤病毒（HPV）感染导致宫颈恶性肿瘤的风险，我们在日本开展了一项病例对照研究。采用一种基于PCR的新检测方法（LCR-E7 PCR），对异常宫颈细胞样本（366份）和正常细胞样本（1562份）进行HPV DNA检测。当考虑单一HPV感染时，在正常宫颈和低级别鳞状上皮内病变（LSIL）中鉴定出26种不同的HPV类型；而在高级别鳞状上皮内病变（HSIL）和宫颈鳞状细胞癌（SCC）中检测到HPV-16、-18、-31、-33、-35、-45、-51、-52、-56、-58和-67，在宫颈腺癌中检测到HPV-16和-18。在尖锐湿疣组织中检测到HPV-6和-11。在HSIL和SCC中，HPV-16是最常见的类型，HPV-51、-52和-58次之；而未检测到HPV-39、-59和-68。通过比值比（OR）分析发现，HPV-11、-39、-42、-44、-53、-59、-62和-66（HPV-66：OR，139；95%置信区间（CI）=6.7-168）与LSIL相关；HPV-16、-18、-31、-51、-52和-58（HPV-16：OR，69；95%CI=36-131）与SCC相关；HPV-16和-18（OR，94；95%CI=28-317）与腺癌相关。多重HPV感染与LSIL（OR，24；95%CI=13-44）、HSIL（OR，16；95%CI=8.4-32）和SCC（OR，8.3；95%CI=3.2-22）相关，尽管其患病率随病变级别降低。所有结果表明，HPV-6和-11是尖锐湿疣相关类型，HPV-16、-18、-31、-51、-52、-58，可能还有-33、-35、-45、-56和-67是高危HPV类型，在日本许多其他类型是与LSIL相关的类型。对临床样本进行HPV分型和多重HPV感染检测，可能作为宫颈细胞异常的替代标志物。
