创伤弧菌败血症大鼠肝组织凋亡相关基因的动态表达及抗菌药物的作用

Dynamic expressions of liver tissue apoptosis-related genes of Vibrio vulnificus sepsis rats and the effects of antibacterial agents.

作者信息

Lu Zhongqiu, Li Mengfang, Liang Huan, Qiu Qiaomeng, Yang Guangtian, Zhou Tieli, Hong Guangliang

机构信息

Department of Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

出版信息

J Huazhong Univ Sci Technolog Med Sci. 2009 Apr;29(2):193-7. doi: 10.1007/s11596-009-0211-4. Epub 2009 Apr 28.

DOI:10.1007/s11596-009-0211-4

PMID:19399403

Abstract

Dynamic changes in mRNA expressions of liver tissue apoptosis-promoting genes Fas and Bax and apoptosis-inhibiting gene Bcl-2 of vibrio vulnificus sepsis rats were detected and the effects of antibacterial agents were examined. The rat model with Vibrio vulnificus sepsis (VV group) was established and some of the Vibrio vulnificus sepsis rats were treated with antibacterial agents (AA group). The mRNA expressions of Fas, Bax and Bcl-2 were measured by reverse transcription polymerase chain reaction (RT-PCR). As compared with normal control group (NC group), the expressions of Fas and Bax mRNA in liver tissue at all different time points in VV group were increased significantly (P<0.05), and the highest levels of Fas and Bax mRNA expressions were 6 and 12 h after the infection, respectively. At the same time, the expression of Bcl-2 mRNA in liver tissue at all different time points in VV group were decreased significantly (P<0.05), and the lowest level of Bcl-2 mRNA expression appeared 2 h after the infection. The mRNA expressions of Bcl-2 in liver tissue 9 and 12 h after the infection in AA group were increased significantly (P<0.05) compared with NC group, while the expressions of Fas and Bax mRNA were not significantly different from those of NC group. Compared with VV group, the expression of Fas mRNA in AA group was decreased (P<0.05) and Bax mRNA was decreased significantly 12 and 16 h after the infection (P<0.05), while the expressions of Bcl-2 mRNA were increased significantly 9, 12 and 16 h after the infection (P<0.05). It is concluded that the mRNA expressions of liver tissue apoptosis-promoting genes Fas and Bax were increased remarkably in vibrio vulnificus sepsis rats, whereas the expression of apoptosis-inhibiting gene Bcl-2 mRNA was decreased obviously in sepsis rats in early stage. The treatment with cefoperazone sodium and levofloxacin lactate could inhibit the expression of Fas mRNA and Bax mRNA and enhance the expression of Bcl-2 mRNA at the same time.

摘要

检测创伤弧菌败血症大鼠肝脏组织促凋亡基因Fas和Bax以及抗凋亡基因Bcl-2的mRNA表达动态变化，并考察抗菌药物的作用。建立创伤弧菌败血症大鼠模型（VV组），部分创伤弧菌败血症大鼠给予抗菌药物治疗（AA组）。采用逆转录聚合酶链反应（RT-PCR）检测Fas、Bax和Bcl-2的mRNA表达。与正常对照组（NC组）比较，VV组各时间点肝脏组织Fas和Bax mRNA表达均显著升高（P<0.05），Fas和Bax mRNA表达最高水平分别在感染后6 h和12 h。同时，VV组各时间点肝脏组织Bcl-2 mRNA表达均显著降低（P<0.05），Bcl-2 mRNA表达最低水平出现在感染后2 h。与NC组比较，AA组感染后9 h和12 h肝脏组织Bcl-2 mRNA表达显著升高（P<0.05），而Fas和Bax mRNA表达与NC组比较差异无统计学意义。与VV组比较，AA组Fas mRNA表达降低（P<0.05），感染后12 h和16 h Bax mRNA表达显著降低（P<0.05），而感染后9 h、12 h和16 h Bcl-2 mRNA表达显著升高（P<0.05）。结论：创伤弧菌败血症大鼠肝脏组织促凋亡基因Fas和Bax的mRNA表达明显升高，而抗凋亡基因Bcl-2 mRNA表达在败血症早期明显降低。头孢哌酮钠和乳酸左氧氟沙星治疗可抑制Fas mRNA和Bax mRNA表达，同时增强Bcl-2 mRNA表达。