Lu Zhong-Qiu, Li Meng-Fang, Liang Huan, Qiu Qiao-Meng, Zhou Tie-Li, Hong Guang-Liang, Liu Gan
Department of Emergency, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
Zhonghua Yi Xue Za Zhi. 2009 Jan 13;89(2):138-41.
To investigate the effects of antimicrobial agents on the Toll like receptors (TLRs) and myeloid differentiation protein (MD)-2 in liver tissue of alcohol-induced liver disease with Vibrio vulnificus (VV) sepsis.
Eighty SD rats were randomly divided into 2 groups: alcohol gastric lavage group (n = 74) undergoing alcohol gastric perfusion once a day for 10 weeks and normal control group (Group N, n = 6). 66 surviving rats in the gastric perfusion group were randomly divided into 6 equal subgroups: Subgroup A was alcohol-induced liver disease control subgroup. Subgroup AA, alcohol-induced liver disease and antibacterial drug control subgroup, underwent intraperitoneal injection of cefoperazone sodium and levofloxacin (LVFX). The other 9 subgroups underwent subcutaneous injection of VV to establish animal model of VV sepsis, the rats of 4 of which were killed 2, 6, 12, and 24 h later respectively with their livers taken out (Subgroups AV), and the rats of 5 of which underwent intraperitoneal injection of cefoperazone sodium and LVFX 4 h after VV injection twice a day (Subgroups AVA) and were killed 6, 12 , 24 , 36 h, and 1 week later with their livers taken out. The behavioral changed were observed. PCR was used to detect the mRNA expression of TLR2, TLR4, and MD-2.
The mRNA expression levels of TLR2, TLR4, and MD-2 in liver 2, 6, 12, and 24 h after in Subgroups AV were all significantly higher than those of Group N (P < 0.05 or P < 0.01) with the maximum level in the AV-12 h subgroup. The mRNA expression levels of TLR2, TLR4, and MD-2 in liver 6 h, 12 h, and 24 h after the VV injection of Subgroup AVA were all significantly lower than those of Group AV (P < 0.05 or P < 0.01).
The mRNA expression levels of TLR2, TLR4, and MD-2 in liver tissue of alcohol-induced liver disease with VV sepsis, which may be reduced by treatment of cefoperazone sodium and LVFX, are associated with the development of VV sepsis. This treatment is effective on this disease. Dynamic monitoring of the mRNA expression levels of TLR2, TLR4, and MD-2 in liver tissue benefits observation of the VV sepsis progress and treatment.
探讨抗菌药物对创伤弧菌（VV）败血症所致酒精性肝病大鼠肝组织中Toll样受体（TLRs）和髓样分化蛋白（MD）-2的影响。
80只SD大鼠随机分为2组：酒精灌胃组（n = 74），每天进行1次酒精灌胃，持续10周；正常对照组（N组，n = 6）。灌胃组66只存活大鼠随机分为6个相等的亚组：A亚组为酒精性肝病对照组。AA亚组为酒精性肝病及抗菌药物对照组，腹腔注射头孢哌酮钠和左氧氟沙星（LVFX）。其他9个亚组皮下注射VV建立VV败血症动物模型，其中4个亚组的大鼠分别于2、6、12和24小时后处死并取出肝脏（AV亚组），另外5个亚组的大鼠在注射VV后4小时腹腔注射头孢哌酮钠和LVFX，每天2次（AVA亚组），并于6、12、24、36小时和1周后处死并取出肝脏。观察行为变化。采用聚合酶链反应（PCR）检测TLR2、TLR4和MD-2的mRNA表达。
AV亚组肝组织在2、6、12和24小时时TLR2、TLR4和MD-2的mRNA表达水平均显著高于N组（P < 0.05或P < 0.01），以AV-12小时亚组最高。AVA亚组在注射VV后6、12和24小时肝组织中TLR2、TLR4和MD-2的mRNA表达水平均显著低于AV亚组（P < 0.05或P < 0.01）。
创伤弧菌败血症所致酒精性肝病大鼠肝组织中TLR2、TLR4和MD-2的mRNA表达水平与创伤弧菌败血症的发生发展有关，头孢哌酮钠和左氧氟沙星治疗可能会降低其表达水平。该治疗方法对该病有效。动态监测肝组织中TLR2、TLR4和MD-2的mRNA表达水平有助于观察创伤弧菌败血症的进展及治疗效果。
