Kulikov Artem U, Galat Marina N
Department of Chemical Metrology, Kharkov V.N. Karazin National University, Kharkov, Ukraine.
J Sep Sci. 2009 May;32(9):1340-50. doi: 10.1002/jssc.200800716.
The paper describes a new test designed in micellar LC (MLC) to compare the commercial C18 stationary phase properties. This test provides the total hydrophobicity, hydrophilicity, steric selectivity, hydrogen bonding, and ion-exchange capacity properties calculation of the ODS stationary phases. Both the test compounds and chromatographic separation conditions choice for column characterization in MLC are detailed. The chromatographic performance of several stationary phases that are used in MLC was evaluated with specific chromatographic test comprising nine test compounds, possessing different physico-chemical properties, which were injected on different supports with two micellar mobile phases: one at pH 7.0 (0.075 mol/L SDS and 1.5% v/v 1-pentanol), and other at pH 2.7 (0.075 mol/L SDS and 1.5% v/v 1-pentanol adjusted to pH by TFA). Fundamental column chromatographic properties were obtained under these conditions and were treated by hierarchical cluster analysis. From the results of cluster analysis, two closely related groups of columns are distinguished, and it was shown that the chosen column characteristic parameters allow characterizing both sorbent and micellar chromatographic system properties. Eleven columns were analyzed by this test, which allows a comparison of columns with the aim of the selection of suitable and analogous column for the analysis with MLC.
该论文描述了一种为比较商用C18固定相性质而设计的胶束液相色谱（MLC）新测试方法。此测试可对ODS固定相的总疏水性、亲水性、空间选择性、氢键作用及离子交换容量性质进行计算。文中详细介绍了用于MLC柱表征的测试化合物及色谱分离条件的选择。采用包含九种具有不同物理化学性质的测试化合物的特定色谱测试方法，在两种胶束流动相条件下，对不同载体上注入的几种用于MLC的固定相的色谱性能进行了评估：一种流动相pH为7.0（0.075 mol/L SDS和1.5% v/v 1 - 戊醇），另一种流动相pH为2.7（0.075 mol/L SDS和1.5% v/v 1 - 戊醇，用TFA调节至pH）。在这些条件下获得了基本的柱色谱性质，并通过层次聚类分析进行处理。从聚类分析结果中，区分出了两组密切相关的柱，结果表明所选择的柱特征参数能够表征吸附剂和胶束色谱系统的性质。通过该测试对11根柱进行了分析，该测试能够对柱进行比较，以便为MLC分析选择合适且类似的柱。
