Tan Dino Bee Aik, Fernandez Sonia, French Martyn, Price Patricia
School of Pathology and Laboratory Medicine, University of Western Australia, Australia.
Clin Immunol. 2009 Jul;132(1):63-70. doi: 10.1016/j.clim.2009.03.518. Epub 2009 Apr 28.
We evaluated NK cell subsets and functions in previously immunodeficient HIV patients responding to ART. Cytokine receptor mRNA was quantitated in purified CD56+ cells. Data were correlated with CD4+ T-cell counts and IFNgamma responses to CMV. NK cell IFNgamma responses to K562 cells and proportions of CD56lo NK cells were correlated in patients (p < 0.001) and both were lower than in controls (p < 0.001 and p = 0.008, respectively), so all patients had poor NK cell function. Proportions of CD56hi NK cells correlated inversely with current CD4+ T-cell counts (p = 0.006) and perforin expression in CD56hi NK cells was higher in patients than controls (p < 0.05). Hence increased proportions and cytolytic function of CD56hi NK cells may partially compensate for CD4+ T-cell deficiency. NK cell IFNgamma responses correlated inversely with expression of IL-10 and IL-12 receptor mRNA. Expression of these transcripts is reduced by exposure to the cytokines, which may reflect immune activation in immunodeficient patients.
我们评估了既往免疫缺陷的HIV患者在接受抗逆转录病毒治疗（ART）时的自然杀伤（NK）细胞亚群及功能。对纯化的CD56⁺细胞中的细胞因子受体信使核糖核酸（mRNA）进行定量分析。数据与CD4⁺T细胞计数以及对巨细胞病毒（CMV）的γ干扰素（IFNγ）反应相关。患者的NK细胞对K562细胞的IFNγ反应与CD56低表达NK细胞比例相关（p<0.001），且两者均低于对照组（分别为p<0.001和p = 0.008），因此所有患者的NK细胞功能均较差。CD56高表达NK细胞比例与当前CD4⁺T细胞计数呈负相关（p = 0.006），且患者CD56高表达NK细胞中的穿孔素表达高于对照组（p<0.05）。因此，CD56高表达NK细胞比例增加及其细胞溶解功能可能部分补偿CD4⁺T细胞缺陷。NK细胞IFNγ反应与白细胞介素10（IL-10）和IL-12受体mRNA的表达呈负相关。暴露于这些细胞因子会使这些转录本的表达降低，这可能反映了免疫缺陷患者的免疫激活情况。
