Fernandez Sonia, Stone Shelley F, Price Patricia, French Martyn A
Department of Clinical Immunology and Immunogenetics, Royal Perth Hospital, Perth, Australia.
Clin Immunol. 2008 Aug;128(2):228-37. doi: 10.1016/j.clim.2008.03.517. Epub 2008 May 29.
Some HIV patients who previously experienced severe immunodeficiency retain low pathogen-specific T-cell responses despite a virological response to antiretroviral therapy (ART). To identify correlates with dysfunction in accessory cell populations, HIV patients were stratified into groups maintaining high or low CD4(+) T-cell IFN-gamma responses to cytomegalovirus (CMV) over 4-8 years on ART. Myeloid dendritic cells (mDC), plasmacytoid (p) DC, M-DC8(+) cells and monocytes were enumerated and mRNA of cytokines and activation molecules were quantitated in purified subpopulations. Proportions of pDC were lower (p=0.043) and mDC were higher (p=0.043) in low responders. TRAIL receptor 2 (DR5) mRNA levels in pDC (p=0.0008) and mDC (p=0.0062) were lower in high responders compared to controls. Levels of IL-15 mRNA were higher in mDC from high responders (p=0.015) and levels of IL-10 mRNA were higher in M-DC8(+) cells from low responders (p=0.036). Hence CMV-specific CD4(+) T-cell IFN-gamma responses may be affected by numbers and function of circulating DC.
一些曾经历严重免疫缺陷的HIV患者，尽管对抗逆转录病毒疗法（ART）有病毒学反应，但仍保持低病原体特异性T细胞反应。为了确定与辅助细胞群体功能障碍相关的因素，将HIV患者分层为在接受ART治疗的4至8年中，对巨细胞病毒（CMV）维持高或低CD4(+) T细胞干扰素-γ反应的组。对髓样树突状细胞（mDC）、浆细胞样（p）DC、M-DC8(+)细胞和单核细胞进行计数，并对纯化亚群中细胞因子和激活分子的mRNA进行定量。低反应者中pDC的比例较低（p = 0.043），mDC的比例较高（p = 0.043）。与对照组相比，高反应者pDC（p = 0.0008）和mDC（p = 0.0062）中TRAIL受体2（DR5）的mRNA水平较低。高反应者mDC中IL-15 mRNA水平较高（p = 0.015），低反应者M-DC8(+)细胞中IL-10 mRNA水平较高（p = 0.036）。因此，CMV特异性CD4(+) T细胞干扰素-γ反应可能受循环DC数量和功能的影响。
