一种建立原位MB49膀胱癌小鼠模型的简单有效方法

[A simple and efficient method for establishing a mouse model of orthotopic MB49 bladder cancer].

作者信息

Liang Zhong-kun, Zhang Lin, Hu Zhi-ming, Chen Zhong, Huang Xin, Shi Xiang-hua, Tan Wan-long, Gao Ji-min

机构信息

Department of Urologic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2009 Apr;29(4):627-30.

PMID:19403380

Abstract

OBJECTIVE

To establish a simple and efficient method for establishing a mouse model of orthotopic superficial bladder cancer.

METHODS

C57BL/6 mice were anesthetized with sodium pentobarbital and catheterized with modified IV catheter (24 G). The mice were intravesically pretreated with HCl and then with NaOH, and after washing the bladders with phosphate-buffered saline (PBS), 100 microl (1 x 10(7)) MB49 cells were infused and allowed to incubate in the bladder for 2 h followed intravesical mitomycin C (MMC) administration. The tumor formation rate, survival, gross hematuria, and bladder weight were determined as the outcome variables, and the pathology of the bladders was observed.

RESULTS

Instillation of MB49 tumor cells resulted in a tumor formation rates of 100% in all the pretreated groups while 0% in the control group without pretreatment. MMC significantly reduced the bladder weight as compared to PBS.

CONCLUSION

We have successfully established a stable, reproducible, and reliable orthotopic bladder cancer model in mice.

摘要

目的

建立一种简单有效的方法来构建原位浅表性膀胱癌小鼠模型。

方法

用戊巴比妥钠麻醉C57BL/6小鼠，并用改良的静脉导管（24G）进行插管。小鼠膀胱内先予盐酸预处理，然后予氢氧化钠预处理，用磷酸盐缓冲盐水（PBS）冲洗膀胱后，注入100微升（1×10⁷）MB49细胞，并使其在膀胱内孵育2小时，随后膀胱内给予丝裂霉素C（MMC）。将肿瘤形成率、生存率、肉眼血尿和膀胱重量作为观察指标，并观察膀胱的病理学变化。