Wyatt R M, Dawson J R
Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.
J Immunol. 1991 Nov 15;147(10):3381-8.
Tonsil B cells were analyzed for their capacity to interact directly with NK cells in vitro. A specific, direct interaction between NK cells and B cells could be detected by direct conjugation and by cold target inhibition using the B lymphoblastoid cell line BJA.B as a labeled target. The data further suggest that the B cell interaction with NK cells specifically activates the NK effectors and induces their production of IFN-gamma. The NK-interactive population of tonsil B cells were characterized as low-buoyant density cells (by Percoll gradient fractionation) that stained more brightly with Hoechst 33342, both characteristics of activated B cells. Immunofluorescent staining of NK cell-B cell conjugates allowed determination of the cell-surface antigenic phenotype of conjugate-forming B cells. B cell targets were ICAM-1bri, 4F2+, TfR+, CD32+, BB1+, and CD77-. They tended to be CD38-, but overlapped the CD38+ population. No correlation was seen with CD37, CD44, CD75, CD76, HC2, or Ig kappa. This phenotype is most consistent with a late activation stage of differentiation, just before and overlapping the expression of CD38. These B cells do not appear significantly sensitive to NK-mediated cytolysis, suggesting that NK cell cytokine synthesis and secretion (e.g., IFN-gamma) may be more important in the NK cell regulation of the humoral response.
对扁桃体B细胞在体外与自然杀伤细胞（NK细胞）直接相互作用的能力进行了分析。通过直接结合以及使用B淋巴母细胞系BJA.B作为标记靶细胞的冷靶抑制，可以检测到NK细胞与B细胞之间存在特异性的直接相互作用。数据进一步表明，B细胞与NK细胞的相互作用特异性地激活了NK效应细胞，并诱导其产生γ干扰素。扁桃体B细胞中与NK细胞相互作用的群体被鉴定为低浮力密度细胞（通过Percoll梯度分级分离），这些细胞用Hoechst 33342染色更明亮，这两个特征均为活化B细胞的特征。对NK细胞 - B细胞结合物进行免疫荧光染色，可以确定形成结合物的B细胞的细胞表面抗原表型。B细胞靶细胞为ICAM - 1高表达、4F2 +、TfR +、CD32 +、BB1 +和CD77 -。它们倾向于CD38 -，但与CD38 +群体有重叠。未发现与CD37、CD44、CD75、CD76、HC2或Ig κ相关。这种表型与分化的晚期激活阶段最为一致，恰好在CD38表达之前并与之重叠。这些B细胞似乎对NK介导的细胞溶解不敏感，这表明NK细胞细胞因子的合成和分泌（例如γ干扰素）在NK细胞对体液反应的调节中可能更为重要。
