Brorson K A, Beverly B, Kang S M, Lenardo M, Schwartz R H
Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD 20892.
J Immunol. 1991 Nov 15;147(10):3601-9.
Cytokines are immunoregulatory proteins that are secreted by T lymphocytes and other cells upon activation. A controversy exists as to whether the induction of cytokine production is mediated at the transcriptional level by the initiation of RNA synthesis or at the post-transcriptional level by the enhancement of mRNA stability. We show that in a nontransformed T cell clone the induction of all of the cytokines that are analyzed in this report is mediated transcriptionally. We also found that a constitutive signal was seen in resting cells when the probes used in the nuclear run-on assay contained some potential cross-hybridizing sequences, for example a poly (A) tract in a cDNA probe. This observation could explain the constitutive signals seen in other studies that claim cytokine production is regulated by differential mRNA stability.
细胞因子是免疫调节蛋白，由T淋巴细胞和其他细胞在激活后分泌。关于细胞因子产生的诱导是在转录水平通过RNA合成的起始介导，还是在转录后水平通过mRNA稳定性的增强介导，存在争议。我们表明，在一个未转化的T细胞克隆中，本报告中分析的所有细胞因子的诱导都是通过转录介导的。我们还发现，当核转录分析中使用的探针包含一些潜在的交叉杂交序列时，例如cDNA探针中的聚（A）序列，在静息细胞中可以看到组成性信号。这一观察结果可以解释其他研究中看到的组成性信号，这些研究声称细胞因子的产生受mRNA稳定性差异的调节。
