McCarthy D, Rehman A, Batchelder D N, Bubel M, Bainbridge D
School of Biological Sciences, Queen Mary and Westfield College, London, U.K.
J Immunol Methods. 1991 Oct 25;143(2):167-73. doi: 10.1016/0022-1759(91)90042-e.
A reflectance measuring instrument (fibre-optic spectrometer linked to a personal computer) which will quantitate the results of immunoassays that result in the deposition of a coloured chromophore on a membrane or other similar solid phase (SP) is described and the reflectance spectra of some chromophores which are commonly produced or used in SP immunoassays are presented. The instrument has been used in conjunction with silver-enhanced SP-immunogold capture assays to determine the concentrations in serum of IgG and of IgM rheumatoid factor (RF) and the results have been compared with those obtained by rate nephelometry and latex agglutination assays, respectively. It is concluded that fibre-optic reflectance photometry is an accurate and useful means of quantitating SP immunoassays in which coloured chromophores are produced and that the results obtained from such immunoassays are comparable to those given by established procedures.
描述了一种反射率测量仪器（与个人计算机相连的光纤光谱仪），它能够对免疫测定结果进行定量，这些免疫测定会导致有色发色团沉积在膜或其他类似的固相（SP）上，并给出了一些在固相免疫测定中常见的发色团的反射光谱。该仪器已与银增强固相免疫金捕获测定法结合使用，以测定血清中IgG和IgM类风湿因子（RF）的浓度，并且分别将结果与通过速率散射比浊法和乳胶凝集试验获得的结果进行了比较。得出的结论是，光纤反射率光度法是对产生有色发色团的固相免疫测定进行定量的准确且有用的方法，并且从这种免疫测定获得的结果与既定程序给出的结果具有可比性。
