Sundaresan P R, De Luca L M
J Natl Cancer Inst. 1977 Jun;58(6):1643-5. doi: 10.1093/jnci/58.6.1643.
Concentrations of retinol and tetinyl esters were assayed in rat intestinal mucosa and in chemically induced transplanted mucinous adenocarcinoma of the jejunum. Lipid extract from the tissues was chromatographed on deactivated alumina to isolate retinol and retinyl esters, which were determined by specific spectrofluorometry. Normal intestinal mucosa tissue contains 556 ng of retinol equivalents as retinyl esters and 303 ng of free retinol/g of wet tissue. The concentration of retinyl esters in the intestinal mucosa from rats carrying the transplanted tumor was 341 ng/g wet tissue; no free retinol was detected in the small intestinal epithelium of these rats. Liver tissue from the tumor-bearing rats contained 157 microng of retinol equivalents as retinyl esters and 136 microng of free retinol/g of wet tissue. The concentration of vitamin A per cell in the adenocarcinoma tissue was about 20 times less than that in intestinal epithelium.
对大鼠肠黏膜以及化学诱导的空肠黏液腺癌组织中的视黄醇和视黄酯浓度进行了测定。将组织中的脂质提取物在失活氧化铝上进行色谱分离，以分离视黄醇和视黄酯，然后通过特定的荧光分光光度法进行测定。正常肠黏膜组织每克湿组织含556纳克视黄醇当量的视黄酯和303纳克游离视黄醇。携带移植瘤大鼠的肠黏膜中视黄酯浓度为每克湿组织341纳克；在这些大鼠的小肠上皮中未检测到游离视黄醇。荷瘤大鼠的肝组织每克湿组织含157微克视黄醇当量的视黄酯和136微克游离视黄醇。腺癌组织中每细胞的维生素A浓度比肠上皮细胞中的低约20倍。
