Henriksen O, Law L W, Appella E
J Natl Cancer Inst. 1977 Jun;58(6):1785-8. doi: 10.1093/jnci/58.6.1785.
Quantitative studies have been performed on the immunogenicity of a membrane-bound antigen of a simian virus 40 (SV40) -induced sarcoma in syngeneic BALB/c mice and of subcellular fractions derived from this tumor. The objectives of the investigation were: a) to develop a quantitative in vivo assay of the tumor-specific transplantation antigen (TSTA) and b) to compare the distribution of histocompatibility antigens, H-2, with that of the SV40 TSTA during several fractionation steps. The immunogenicity of the TSTA-containing fractions was assessed from dose-response curves relating tumor size and the amount of protein used for immunization. After digestion of the tumor cell membranes with a limited amount of papain, H-2 as well as TSTA were present in a soluble form. A single immunization with only 2 microng of the solubilized TSTA reduced the tumor size by 70% compared to that in nonimmunized control animals. The results of several fractionation steps suggest that H-2 and the TSTA are not tightly associated in the solubilized immunogenic material.
已对猴病毒40（SV40）诱导的肉瘤的膜结合抗原在同基因BALB/c小鼠中的免疫原性以及源自该肿瘤的亚细胞组分进行了定量研究。该研究的目的是：a）开发一种肿瘤特异性移植抗原（TSTA）的定量体内测定方法，以及b）在几个分级分离步骤中比较组织相容性抗原H-2与SV40 TSTA的分布。从与肿瘤大小和用于免疫的蛋白量相关的剂量反应曲线评估含TSTA组分的免疫原性。用有限量的木瓜蛋白酶消化肿瘤细胞膜后，H-2以及TSTA以可溶形式存在。与未免疫的对照动物相比，仅用2微克溶解的TSTA进行一次免疫可使肿瘤大小减少70%。几个分级分离步骤的结果表明，在溶解的免疫原性物质中，H-2和TSTA没有紧密结合。
