Zhang Li-Hua, Bai Chao-Hong, Wang Ying-Song, Jiang Yu-Cheng, Hu Man-Cheng, Li Shu-Ni, Zhai Quan-Guo
School of Chemistry and Materials Science, Shaanxi Normal University, Chang'an South Road, 199, Xi'an, Shaanxi, 710062, People's Republic of China.
Biotechnol Lett. 2009 Aug;31(8):1269-72. doi: 10.1007/s10529-009-0009-2. Epub 2009 Apr 29.
Chloroperoxidase (CPO) from Caldariomyces fumago was optimally covalently immobilized on chitosan membranes pretreated with 0.8 M glutaraldehyde at pH 3.5 to give 3.18 mg CPO g(-1) support. Using monochlorodimedone (MCD) as assay substrate, the immobilized-CPO retained 40% activity at 50 degrees C after 40 min whereas free CPO retained only 0.02%. The residual activity for immobilized-CPO was 99 and 58% compared with 68 and 43% for free CPO in the presence of 1.5 M urea and 300 microM H(2)O(2), respectively, after 20 h.
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