Kaup Bjoern-Arne, Ehrich Kristina, Pescheck Michael, Schrader Jens
DECHEMA e.V, Karl-Winnacker-Institut, Biochemical Engineering Group, Theodor-Heuss-Allee 25, 60486 Frankfurt, Germany.
Biotechnol Bioeng. 2008 Feb 15;99(3):491-8. doi: 10.1002/bit.21713.
Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Particles of approximately 500 microm in diameter had no effect on growth morphology or productivity of CPO formation by C. fumago. In contrast particles of < or =42 microm in diameter led to the dispersion of the C. fumago mycelia up to the level of single hyphae. Under these conditions the maximum specific productivity of CPO formation was enhanced about fivefold and an accumulated CPO activity in the culture supernatant of more than 1,000 U mL(-1) was achieved after 10-12 days of cultivation. In addition, the novel cultivation method also showed a positive effect on growth characteristics of other filamentous microorganisms proven by the stimulation of single hyphae/cell formation.
微粒增强培养（MPEC）被用作一种新方法，以在丝状微生物培养过程中提高生物量和产物形成。例如，在存在和不存在不同大小微粒的情况下，分析了烟曲霉产生氯过氧化物酶（CPO）的情况。直径约500微米的颗粒对烟曲霉的生长形态或CPO形成的生产力没有影响。相比之下，直径小于或等于42微米的颗粒导致烟曲霉菌丝体分散至单根菌丝水平。在这些条件下，CPO形成的最大比生产力提高了约五倍，培养10 - 12天后，培养上清液中的CPO累积活性达到超过1000 U mL(-1)。此外，这种新的培养方法对其他丝状微生物的生长特性也显示出积极影响，这通过单根菌丝/细胞形成的刺激得到证明。
